Persistent viral infections impact key biological traits in Drosophila melanogaster.

IF 7.2 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2025-10-09 DOI:10.1371/journal.pbio.3003437

Mauro Castelló-Sanjuán, Rubén González, Ottavia Romoli, Hervé Blanc, Jared C Nigg, Maria-Carla Saleh

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引用次数: 0

Abstract

Persistent viral infections have been assumed to impose minimal fitness costs for insects. We established persistent mono-infections of Drosophila melanogaster with four different enteric RNA viruses: Drosophila A virus (DAV), Drosophila C virus (DCV), Bloomfield virus, and Nora virus. We observed that these infections significantly reduce fly survival, alter the number of viable offspring per female, modulate microbiome composition, impact locomotor abilities, and change activity patterns. These results demonstrate the significant impact of persistent viral infections on key biological traits and expand our understanding of the fitness costs of persistent viral infections for the host. In addition, the four viruses displayed different accumulation kinetics and elicited unique transcriptional profiles with no common core responses. The transcriptional changes triggered by DCV infection persisted even after viral clearance. This comprehensive comparative dataset represents a valuable resource for researchers studying host-pathogen interactions, providing detailed transcriptional profiles, and behavioral measurements across different viral infections and time points. Our findings reveal that persistent viral infections modulate critical aspects of insect biology, affecting host physiology and behavior.

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持续的病毒感染影响黑腹果蝇的关键生物学性状。

持续的病毒感染被认为对昆虫施加最小的适应成本。我们建立了四种不同的肠道RNA病毒：果蝇A病毒（DAV）、果蝇C病毒（DCV）、布卢姆菲尔德病毒和诺拉病毒对黑腹果蝇的持续单感染。我们观察到，这些感染显著降低了苍蝇的存活率，改变了每只雌性可存活后代的数量，调节了微生物组组成，影响了运动能力，并改变了活动模式。这些结果证明了持续病毒感染对关键生物学性状的显著影响，并扩展了我们对持续病毒感染对宿主适应性成本的理解。此外，这四种病毒表现出不同的积累动力学，并引发了独特的转录谱，没有共同的核心反应。由DCV感染引发的转录变化即使在病毒清除后仍持续存在。这个全面的比较数据集为研究宿主-病原体相互作用的研究人员提供了宝贵的资源，提供了详细的转录谱，以及不同病毒感染和时间点的行为测量。我们的研究结果表明，持续的病毒感染调节昆虫生物学的关键方面，影响宿主的生理和行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

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