Tumor potential biomarker: ferritin heavy chain promotes proliferation and migration of oral squamous cell carcinoma

IF 1.9 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2026-03-01 Epub Date: 2025-09-17 DOI:10.1016/j.oooo.2025.09.012

Wenxue Huang , Menglong Hu , Ye Zhang , Yan Han , Jianxia Hou

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引用次数: 0

Abstract

Objective

Oral squamous cell carcinoma (OSCC) is one of common malignancy. Ferritin heavy chain (FTH), plays a crucial role in iron homeostasis and regulates cell proliferation. The aims of this study are to investigate the functions of FTH in the progression of OSCC.

Study Design

This study included twenty OSCC tissues and adjacent non-tumor tissue samples collected from patients who were pathologically diagnosed with OSCC. We detected expression of FTH gene by qRT-PCR. Meanwhile, expression of marker Ki67, cytokeratin, EGFR, P53, P63, and P40 were detected by immunohistochemistry. Cell proliferation assay, wound healing assay, qPCR and western blot were measured after knockdown of the FTH by small interfering RNAs.

Results

Data from the investigation showed that FTH was highly expressed in OSCC tissues. The location of the expression is proliferating epithelial cells, correlated well with the location of Ki67 and P63. Knockdown of FTH inhibited proliferation, migration and invasion of SCC15 and CAL27 cell lines. Adhesion molecules including E-Cadherin, Claudin 1, and Collagen were up-regulation after knockdown of FTH.

Conclusion

FTH is overexpressed in OSCC tissues and promotes tumor cell proliferation and invasion. It may be a novel biomarker and therapeutic target in prognosis and treatment of patients with OSCC.

查看原文本刊更多论文

肿瘤潜在生物标志物铁蛋白重链促进口腔鳞状细胞癌的增殖和迁移。

目的：口腔鳞状细胞癌是口腔常见的恶性肿瘤之一。铁蛋白重链（FTH）在铁稳态和细胞增殖中起重要作用。本研究的目的是探讨FTH在OSCC进展中的功能。研究设计：本研究包括从病理诊断为OSCC的患者中收集的20个OSCC组织和邻近的非肿瘤组织样本。采用qRT-PCR检测FTH基因的表达。同时免疫组化检测标志物Ki67、细胞角蛋白、EGFR、P53、P63、P40的表达。小干扰rna敲除FTH后进行细胞增殖试验、创面愈合试验、qPCR和western blot检测。结果：调查数据显示，FTH在OSCC组织中高表达。表达位置为增殖上皮细胞，与Ki67和P63的表达位置密切相关。抑制FTH可抑制SCC15和CAL27细胞系的增殖、迁移和侵袭。FTH下调后，粘附分子E-Cadherin、Claudin 1、Collagen上调。结论：FTH在OSCC组织中过表达，促进肿瘤细胞增殖和侵袭。它可能是一个新的生物标志物和治疗靶点的预后和治疗的OSCC患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.