Integrative analysis of serum microorganisms and serum metabolomics in osteoporosis patients based on 16S rDNA sequencing and UHPLC/MS-based metabolomics.
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引用次数: 0
Abstract
Introduction: Although significant progress has been made in the treatment and research of osteoporosis patients in recent years, the genetic mechanism of osteoporosis has not yet been fully elucidated.
Methods: We conducted a comprehensive analysis using 16S sequencing and UHPLC-MS/MS metabolomics data to characterize the microbial composition and metabolic composition in the serum of osteoporosis patients.
Results: At the phylum level, Proteobacteria are mainly present in Osteoporosis; In Normal, it is mainly Bacteroidota. At the genus level, Cupriavidus is the main species in Osteoporosis; In Normal, the main ones are Blautia, Bacteroides, Alcaligenes and Pseudomonas. Serum metabolomics revealed different metabolites (230 significantly differentially expressed metabolites) and lipid metabolism pathways (such as Glycerophospholipid metabolism) among the two groups. The combined serum microbiota and serum metabolomics datasets demonstrate a correlation reflecting the impact of microbiota on metabolic activity (p < 0.05).
Discussion: Our research findings indicate that microbiota and metabolomics analysis provide important candidate biomarkers. The correlation between these serum microbiota and host metabolism is of great significance for optimizing early diagnosis and developing personalized treatment strategies. This study elucidates the relationship between serum microbiota and metabolites in osteoporosis.
期刊介绍：
Frontiers in Medicine publishes rigorously peer-reviewed research linking basic research to clinical practice and patient care, as well as translating scientific advances into new therapies and diagnostic tools. Led by an outstanding Editorial Board of international experts, this multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
In addition to papers that provide a link between basic research and clinical practice, a particular emphasis is given to studies that are directly relevant to patient care. In this spirit, the journal publishes the latest research results and medical knowledge that facilitate the translation of scientific advances into new therapies or diagnostic tools. The full listing of the Specialty Sections represented by Frontiers in Medicine is as listed below. As well as the established medical disciplines, Frontiers in Medicine is launching new sections that together will facilitate
- the use of patient-reported outcomes under real world conditions
- the exploitation of big data and the use of novel information and communication tools in the assessment of new medicines
- the scientific bases for guidelines and decisions from regulatory authorities
- access to medicinal products and medical devices worldwide
- addressing the grand health challenges around the world
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