Nicolas Bailly, Clemens Grünwald-Gruber, Marie Peeters, François Chaumont, Catherine Navarre
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引用次数: 0
Abstract
Nicotiana tabacum BY-2 cell suspension cultures are a powerful platform for producing recombinant glycoproteins such as immunoglobulins. Extensive efforts have been devoted to engineering the N-glycosylation pathway of BY-2 cells to overcome differences between mammalian and plant N-glycans. However, the mucin-type O-glycosylation pathway is absent in plant cells. This modification, which consists of glycan attachment to serine or threonine residues, is important in many human proteins, but is also highly complex. In this regard, plants offer a unique opportunity to engineer this pathway de novo without the interplay of many competing enzymes. In this study, transgenic BY-2 cell lines expressing the enzymes responsible for the formation of Core 1 and Tn antigen glycans were generated. First, GalNAc-O-glycosylation was initiated by the expression of human GalNAcT2. This O-glycan was then elongated by co-expression of Drosophila C1GalT1 to form the core 1 structure. Human IgA1 was produced in these engineered BY-2 cell lines and the presence of mucin-type O-glycans was confirmed by lectin blotting. The precise O-glycosylation profile of the hinge region was determined by mass spectrometry and showed the almost complete disappearance of pentoses and the presence of core 1 O-glycans.
期刊介绍:
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.