{"title":"Comparative aptamer screening for patulin and the development of sensitive colorimetric assay for fruit juice analysis","authors":"Haijun Pan, Ruijing Liu, Dongjie Liu, Lukai Ma, Qin Wang, Gengsheng Xiao, Feng Wang","doi":"10.1007/s00604-025-07591-2","DOIUrl":null,"url":null,"abstract":"<div><p> A gold nanoparticle-based colorimetric assay was developed for patulin, a mycotoxin, detection in fruit juice. Eight patulin-specific aptamers were rigorously screened using molecular docking and specific binding analyses to select the optimal candidate. The optimized assay exhibited a wide linear detection range of 5.0–200.0 ng/mL in the buffer reaction system and high specificity for patulin. Its reliability for real-world samples was validated by achieving good recoveries (83.6%-90.7%) in spiked apple and hawthorn juice, consistent with HPLC results. This study highlights an effective methodology for screening high-performance aptamers for food contaminants and a novel strategy for high-throughput food safety assessment targeting patulin.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07591-2","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
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Abstract
A gold nanoparticle-based colorimetric assay was developed for patulin, a mycotoxin, detection in fruit juice. Eight patulin-specific aptamers were rigorously screened using molecular docking and specific binding analyses to select the optimal candidate. The optimized assay exhibited a wide linear detection range of 5.0–200.0 ng/mL in the buffer reaction system and high specificity for patulin. Its reliability for real-world samples was validated by achieving good recoveries (83.6%-90.7%) in spiked apple and hawthorn juice, consistent with HPLC results. This study highlights an effective methodology for screening high-performance aptamers for food contaminants and a novel strategy for high-throughput food safety assessment targeting patulin.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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