Non-thermal plasma-mediated inactivation of biofilms formed by imipenem-resistant Klebsiella pneumoniae: a non-antibiotic approach

IF 2.9 3区物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY

The European Physical Journal Plus Pub Date : 2025-10-09 DOI:10.1140/epjp/s13360-025-06933-6

Mohammed F. Al-Marjani, Raghad S. Mohammed, Nodira Azimova, Jamoliddin Razzokov, Sanaa Ali Hamza

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Abstract

Bacteria often form complex communities known as biofilms—hydrated extracellular polymeric matrices that protect sessile cells. Non-thermal plasma (NTP) is emerging as a potential strategy for biofilm disruption due to the oxidative damage caused by reactive oxygen and nitrogen species (RONS). In this study, the antimicrobial activity of an argon-based atmospheric pressure NTP jet was evaluated against imipenem-resistant Klebsiella pneumoniae biofilms in vitro. Plasma exposure times ranged from 0 to 120 s. A 15-s treatment reduced bacterial growth by 72% (p < 0.0001), with complete inhibition observed reach 100% at 60–120 s. Biofilm degradation reached 42% at 15 s and 100% at 60 s. Scanning electron microscopy revealed significant morphological damage, including cell deformation and shrinkage. These findings highlight NTP’s potential as an effective, non-antibiotic method for combating multidrug-resistant K. pneumoniae infections.

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非热等离子体介导的亚胺培南耐药肺炎克雷伯菌形成的生物膜失活：一种非抗生素方法

细菌经常形成复杂的群落，被称为生物膜-水合的细胞外聚合物基质，保护无根细胞。由于活性氧和活性氮（RONS）引起的氧化损伤，非热等离子体（NTP）正成为生物膜破坏的潜在策略。在本研究中，研究了氩基常压NTP射流对亚胺培南耐药肺炎克雷伯菌生物膜的体外抗菌活性。等离子体暴露时间从0到120秒不等。15-s处理可使细菌生长减少72% (p < 0.0001)， 60-120 s完全抑制达到100%。15 s生物膜降解率为42%，60 s生物膜降解率为100%。扫描电镜显示明显的形态学损伤，包括细胞变形和收缩。这些发现突出了国家结核控制项目作为对抗多药耐药肺炎克雷伯菌感染的有效、非抗生素方法的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The European Physical Journal Plus PHYSICS, MULTIDISCIPLINARY-

CiteScore

5.40

自引率

8.80%

发文量

1150

审稿时长

4-8 weeks

期刊介绍： The aims of this peer-reviewed online journal are to distribute and archive all relevant material required to document, assess, validate and reconstruct in detail the body of knowledge in the physical and related sciences. The scope of EPJ Plus encompasses a broad landscape of fields and disciplines in the physical and related sciences - such as covered by the topical EPJ journals and with the explicit addition of geophysics, astrophysics, general relativity and cosmology, mathematical and quantum physics, classical and fluid mechanics, accelerator and medical physics, as well as physics techniques applied to any other topics, including energy, environment and cultural heritage.