High-performance liquid chromatography analysis of phenolics in Casuarina cunninghamiana: extraction, quantification and antioxidant evaluation.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-10-09 DOI:10.1080/14786419.2025.2569804

Nourhene Aroua, Maher Boukhris, Wissal Ayouni, Sirine Choura, Mohamed Chamkha, Ridha Ben Salem, Rigane Ghayth

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Abstract

The study was carried out to evaluate the phenolic compounds and antioxidant activity in the aqueous extract from leaves, stems and flowers of Casuarina cunninghamiana Miq. subsp. Cunninghamiana through ultrasound-assisted and maceration extractions from Sfax, Tunisia. The leaves exhibited the highest total phenolic and flavonoid contents. HPLC analysis identified 14 phenolic compounds across all extracts, including caffeic acid, epicatechin and verbascoside. Remarkably, catechin was present at the highest concentration in C. cunninghamiana flowers extracts. The antioxidant activity was assessed using the DPPH radical scavenging assay and FRAP assay. The leaves extract exhibited the strongest antioxidant activity, with an IC₅₀ value of 204.17 µg/mL in the DPPH assay and 197.5 µM BHT/g DW in the FRAP assay. These findings highlight the potential of C. cunninghamiana being studied for the first time in Tunisia as a natural source of bioactive compounds, with potential applications in biotechnology, pharmacology and natural product research.

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木麻黄酚类物质的高效液相色谱分析：提取、定量及抗氧化评价。

对木麻黄（Casuarina cunninghamiana Miq）叶、茎、花水提物中的酚类化合物及其抗氧化活性进行了研究。无性系种群。在突尼斯的斯法克斯，通过超声波辅助和浸渍提取坎宁哈米娜。总酚和类黄酮含量最高。HPLC分析在所有提取物中鉴定出14种酚类化合物，包括咖啡酸、表儿茶素和毛蕊花苷。儿茶素在杉木花提取物中的含量最高。采用DPPH自由基清除法和FRAP法测定其抗氧化活性。其抗氧化活性最强，DPPH IC50值为204.17µg/mL， FRAP IC50值为197.5µM BHT/g DW。这些发现突出了cunninghamiana在突尼斯首次作为生物活性化合物的天然来源进行研究的潜力，在生物技术、药理学和天然产物研究中具有潜在的应用前景。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.