Streptococcus mutans Growth Inhibition by the Probiotic Lacticaseibacillus rhamnosus GG as an Additive to Infant Formula.

IF 1.9 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

International journal of paediatric dentistry Pub Date : 2025-10-08 DOI:10.1111/ipd.70041

Berenice Cheng, Jung-Wei Chen, Zhe Zhong, Jungyi Liu

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引用次数: 0

Abstract

Probiotics have demonstrated Streptococcus mutans (SM) suppression. This in vitro study compared the SM inhibition capacity of bovine infant formula supplemented with Lacticaseibacillus rhamnosus Gorbach Goldin (LGG) probiotic to a plain formulation.

Methods: Two bovine-based infant formulas, Similac Advance Optigro (formula non-probiotic, FNP) and Enfamil Nutramigen LGG (formula with probiotic, FWP) were compared. The formulas were reconstituted at different concentrations (0.1×, 0.5×, 1×, and 2×). LGG (ATCC BAA-3227) suspensions were used as positive controls. All assays were inoculated with SM, spiral plated (n = 8) onto selective Mitis Salivarius Agar plates, and incubated at 37°C for 96 h. SM growth was enumerated in colony-forming units (CFU). Multivariate analysis of variance was performed to assess concentration-dependent effects and statistical significance (α = 0.05).

Results: FWP recorded less SM growth than FNP at the 0.5×, 1.0×, and 2.0× concentrations (ANOVA p < 0.001). FWP concentration was negatively correlated to SM growth (p < 0.001, r = -0.864), whereas FNP showed strong positive trends (p < 0.001, r = 0.821). MANOVA tests confirmed that both group and concentration affect SM growth (p < 0.001).

Conclusion: Commercial probiotic-enriched infant formula effectively suppresses SM growth in vitro. The observed suppression of SM growth in this study provides evidence for this dietary intervention's anticariogenic potential.

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鼠李糖乳杆菌GG益生菌对婴儿配方奶粉中变形链球菌生长的抑制作用。

益生菌已显示出变形链球菌（SM）的抑制作用。本研究比较了添加鼠李糖乳杆菌戈尔巴赫金（LGG）益生菌的牛婴儿配方奶粉与普通配方奶粉对SM的抑制能力。方法：比较Similac Advance Optigro（非益生菌配方，FNP）和Enfamil Nutramigen LGG（含益生菌配方，FWP）两种牛源配方奶粉。分别以不同浓度（0.1×、0.5×、1×、2×）对配方进行复配。LGG （ATCC BAA-3227）悬液作为阳性对照。所有实验用SM接种，螺旋镀（n = 8）于选择性的Mitis Salivarius琼脂板上，37℃孵育96 h。以菌落形成单位（CFU）为单位枚举SM生长。采用多因素方差分析评价浓度依赖性效应及统计学显著性（α = 0.05）。结果：在0.5倍、1.0倍和2.0倍浓度下，FWP的SM生长低于FNP（方差分析p）。结论：商业益生菌婴儿配方奶粉能有效抑制SM的体外生长。本研究中观察到的SM生长抑制为这种饮食干预的抗肿瘤潜力提供了证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International journal of paediatric dentistry 医学-小儿科

CiteScore

5.50

自引率

2.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The International Journal of Paediatric Dentistry was formed in 1991 by the merger of the Journals of the International Association of Paediatric Dentistry and the British Society of Paediatric Dentistry and is published bi-monthly. It has true international scope and aims to promote the highest standard of education, practice and research in paediatric dentistry world-wide. International Journal of Paediatric Dentistry publishes papers on all aspects of paediatric dentistry including: growth and development, behaviour management, diagnosis, prevention, restorative treatment and issue relating to medically compromised children or those with disabilities. This peer-reviewed journal features scientific articles, reviews, case reports, clinical techniques, short communications and abstracts of current paediatric dental research. Analytical studies with a scientific novelty value are preferred to descriptive studies. Case reports illustrating unusual conditions and clinically relevant observations are acceptable but must be of sufficiently high quality to be considered for publication; particularly the illustrative material must be of the highest quality.