Xiaojing Li, Jieshu Li, Hao Liu, Shaojun Yu, Yonggang Liu
{"title":"Isolation, structural characterization, anti-tumor activity, and mechanisms of novel compounds from the roots of Aconitum pendulum Busch","authors":"Xiaojing Li, Jieshu Li, Hao Liu, Shaojun Yu, Yonggang Liu","doi":"10.1016/j.fitote.2025.106923","DOIUrl":null,"url":null,"abstract":"<div><div>Twelve compounds were isolated from the roots of <em>Aconitum pendulum</em> Busch, including a novel alkaloid (compound <strong>1</strong>) and a new lignan (compound <strong>2</strong>), along with ten known compounds. Activity studies demonstrated that compound <strong>2</strong> exhibited the most significant inhibitory effect on two types of tumor cells, HepG2 and HGC-27, with IC<sub>50</sub> values of 6.77 ± 0.34 μM and 2.54 ± 0.16 μM, respectively, surpassing the positive control, 5-fluorouracil. Mechanistic studies indicated that compound <strong>2</strong> exerted anti-tumor effects through multiple pathways, including the inhibition of cell migration, induction of apoptosis, and suppression of colony formation. Network pharmacological analyses suggested that compound <strong>2</strong> exerted multi-targeted anti-tumor effects by modulating various signaling pathways, including HIF-1α and NF-κB. Furthermore, Western blot experiments confirmed that this compound could downregulate HIF-1α expression in a concentration-dependent manner.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"187 ","pages":"Article 106923"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0367326X25005490","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
Abstract
Twelve compounds were isolated from the roots of Aconitum pendulum Busch, including a novel alkaloid (compound 1) and a new lignan (compound 2), along with ten known compounds. Activity studies demonstrated that compound 2 exhibited the most significant inhibitory effect on two types of tumor cells, HepG2 and HGC-27, with IC50 values of 6.77 ± 0.34 μM and 2.54 ± 0.16 μM, respectively, surpassing the positive control, 5-fluorouracil. Mechanistic studies indicated that compound 2 exerted anti-tumor effects through multiple pathways, including the inhibition of cell migration, induction of apoptosis, and suppression of colony formation. Network pharmacological analyses suggested that compound 2 exerted multi-targeted anti-tumor effects by modulating various signaling pathways, including HIF-1α and NF-κB. Furthermore, Western blot experiments confirmed that this compound could downregulate HIF-1α expression in a concentration-dependent manner.
期刊介绍:
Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas:
1. Characterization of active ingredients of medicinal plants
2. Development of standardization method for bioactive plant extracts and natural products
3. Identification of bioactivity in plant extracts
4. Identification of targets and mechanism of activity of plant extracts
5. Production and genomic characterization of medicinal plants biomass
6. Chemistry and biochemistry of bioactive natural products of plant origin
7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.