Description of Tateyamaria algicola sp. nov. and Tateyamaria rhodophyticola sp. nov., Isolated from Marine Algae.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-10-09 DOI:10.1007/s00284-025-04537-y

Byeong Jun Choi, Jeong Min Kim, Hülya Bayburt, Dae Gyu Choi, Seo Hui Choi, Che Ok Jeon

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引用次数: 0

Abstract

Two aerobic, non-motile, Gram-stain-negative strains, designated SN3-11ᵀ and SN6-1ᵀ, were isolated from marine algae. Both strains were oxidase- and catalase-positive, with rod-shaped morphology. Strain SN3-11ᵀ grew optimally at 25 ℃, pH 7.0-8.0, and 2.0-5.0% (w/v) NaCl, while strain SN6-1ᵀ showed optimal growth at 25 ℃, pH 8.0, and 2.0-3.0% NaCl. Both strains contained ubiquinone-10 as the sole respiratory quinone, and their major fatty acids were summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c), C_16:0, and C_19:0 cyclo ω8c. Phosphatidylcholine and phosphatidylglycerol were dominant polar lipids, with diphosphatidylglycerol additionally present in SN3-11ᵀ. Strains SN3-11ᵀ and SN6-1ᵀ had genome sizes of 4,762 Kb and 4,157 Kb with G + C contents of 61.3% and 62.0%, respectively. They shared 96.9% 16S rRNA gene sequence similarity, 77.0% average nucleotide identity (ANI), and 20.2% digital DNA-DNA hybridization (dDDH), supporting their classification as distinct species. Their closest relative, Tateyamaria armeniaca KMU-156ᵀ, exhibited 16S rRNA gene sequence similarities of 98.6% to SN3-11ᵀ and 97.7% to SN6-1ᵀ. Phylogenetic trees based on 16S rRNA and whole-genome sequences placed both strains in distinct lineages within Tateyamaria. ANI and dDDH values between the two isolates and other Tateyamaria species were < 78.5% and < 21.0%, respectively. Strains SN3-11ᵀ and SN6-1ᵀ harbored genes encoding diverse carbohydrate-active enzymes and biosynthetic pathways for lycopene, pantothenate, and riboflavin, suggesting potential symbiotic roles with algal hosts. Based on phenotypic, chemotaxonomic, and genomic characteristics, SN3-11ᵀ and SN6-1ᵀ represent two novel species, Tateyamaria algicola sp. nov. (SN3-11ᵀ = KACC 23689ᵀ = JCM 36649ᵀ) and Tateyamaria rhodophyticola sp. nov. (SN6-1ᵀ = KACC 23127ᵀ = JCM 35962ᵀ).

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海藻中分离的褐藻和红藻褐藻的描述。

从海藻中分离出两株需氧、非运动、革兰氏阴性菌株SN3-11和SN6-1。两株菌株均为氧化酶和过氧化氢酶阳性，呈棒状。菌株SN3-11在25℃、pH 7.0-8.0和2.0-5.0% (w/v) NaCl条件下生长最优，菌株SN6-1在25℃、pH 8.0和2.0-3.0% NaCl条件下生长最优。两菌株均以泛醌-10为唯一呼吸醌，其主要脂肪酸特征分别为8 （C18:1 ω7c和/或C18:1 ω6c）、C16:0和C19:0 cyclo ω8c。磷脂酰胆碱和磷脂酰甘油是主要的极性脂质，SN3-11等位基因中还存在二磷脂酰甘油。菌株SN3-11和SN6-1的基因组大小分别为4,762 Kb和4,157 Kb， G + C含量分别为61.3%和62.0%。它们具有96.9%的16S rRNA基因序列相似性，77.0%的平均核苷酸同源性（ANI）和20.2%的数字DNA-DNA杂交（dDDH），支持它们作为不同物种的分类。它们最近的亲缘种——亚美尼亚Tateyamaria armeniaca KMU-156等位基因，其16S rRNA基因序列与SN3-11等位基因的相似性为98.6%，与SN6-1等位基因的相似性为97.7%。基于16S rRNA和全基因组序列的系统发育树将这两个菌株置于不同的Tateyamaria谱系中。两株菌株与其他菌株间的ANI和dDDH值比较

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.