Diagnostic performance of lateral flow antigen tests versus latex agglutination for cryptococcosis in dogs and cats.

IF 1.4 3区农林科学 Q2 VETERINARY SCIENCES

American journal of veterinary research Pub Date : 2025-10-08 DOI:10.2460/ajvr.25.06.0227

Samantha A Wong, John Morton, Luisa Monteiro de Miranda, Mark B Krockenberger, Elissa Shinozaki, Richard Malik

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引用次数: 0

Abstract

Objective: To investigate the diagnostic utility of 2 point-of-care cryptococcal antigen (CrAg) lateral flow assays (LFAs; Dynamiker Biotechnology Co Ltd and IMMY) for the diagnosis of cryptococcosis in dogs and cats using a latex CrAg agglutination test (Cryptococcal Antigen Latex Agglutination System; Meridian Bioscience) as the reference standard.

Methods: Serum samples from 21 dogs and cats considered to be infected with cryptococcus and 30 animals considered unlikely to be infected were assayed using both point-of-care LFAs from January 2024 through April 2025, and sensitivity and specificity estimates were calculated.

Results: The CrAg LFA (IMMY) had an estimated sensitivity of 95% (95% CI, 76.0% to 100%) and an estimated specificity of 100% (95% CI, 88.0% to 100%). The CrAg LFA (Dynamiker Biotechnology Co Ltd) had an estimated sensitivity of 100% (95% CI, 83.0% to 100%) and specificity of 20% (95% CI, 8.00% to 39.0%).

Conclusions: The CrAg LFA (IMMY) is unequivocally superior to the CrAg LFA (Dynamiker Biotechnology Co Ltd) for the screening of cryptococcosis in dogs and cats due to its vastly superior specificity.

Clinical relevance: The CrAg LFA (Dynamiker Biotechnology Co Ltd) in its present form should not be considered as a useful test to screen cats and dogs for cryptococcosis.

查看原文本刊更多论文

侧流抗原试验与乳胶凝集对犬猫隐球菌病的诊断性能。

目的：探讨2点护理隐球菌抗原（CrAg）横向流动试验（LFAs， Dynamiker生物技术有限公司和IMMY）对犬猫隐球菌病的诊断价值，并以乳胶CrAg凝集试验（cryptococcal antigen latex agglutination System, Meridian Bioscience）为参考标准。方法：对2024年1月至2025年4月期间21只被认为感染隐球菌的狗和猫以及30只被认为不太可能感染的动物的血清样本进行检测，并计算敏感性和特异性。结果：CrAg LFA （IMMY）的估计敏感性为95% (95% CI, 76.0% ~ 100%)，估计特异性为100% （95% CI, 88.0% ~ 100%）。CrAg LFA （Dynamiker Biotechnology Co Ltd）的估计灵敏度为100% (95% CI， 83.0%至100%)，特异性为20% （95% CI， 8.00%至39.0%）。结论：CrAg LFA （IMMY）在狗和猫的隐球菌病筛查方面明显优于CrAg LFA （Dynamiker生物技术有限公司），因为其特异性大大优于CrAg LFA （Dynamiker生物技术有限公司）。临床相关性：目前形式的CrAg LFA （Dynamiker生物技术有限公司）不应被视为筛选猫和狗隐球菌病的有用测试。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.