Chitosan Nanofibrous Dressing Increased Angiogenesis and Anti-inflammatory Response in an Acute Wound Model in Rats: A Comparative Study.

IF 5.4 2区医学 Q3 ENGINEERING, BIOMEDICAL

Annals of Biomedical Engineering Pub Date : 2025-10-08 DOI:10.1007/s10439-025-03842-8

Yury Salkovskiy, Mahboubeh Ghanbari, Carlos P Jara, Sara Cartwright, Pinaki Mondal, Colman Freel, Sayed Ahmadreza Razian, Jason MacTaggart, Mark A Carlson

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Abstract

Purpose: Chitosan-based materials are promising for wound healing because of their antibacterial efficacy, biocompatibility, and biodegradability, though their healing mechanisms remain unclear. This study explores the cellular and molecular mechanisms of wound healing with chitosan nanofibrous dressings.

Methods: Thirty-nine Sprague Dawley rats were divided into three groups based on the type of dressings administered: (1) chitosan nanofibrous dressing + transparent film, (2) commercial scaffold + transparent film, and (3) transparent film alone (control). Full-thickness wounds (2 cm × 2 cm) were created on the dorsum, splinted, and covered with dressings. Evaluations at 7, 14, and 21 days included histological analysis, and measurements of TNF-α and iNOS levels in the wounds.

Results: On day 21, epithelialization was significantly higher in the chitosan group than in the scaffold group (87.5% vs. 42.0%, p = 0.03). TNF-α levels were lower in both treatment groups compared to the controls. In the chitosan group, the CD68+/CD163+ ratio was lower than in the scaffold group (0.28 vs. 0.62, p = 0.037), and blood vessel formation was greater than in the controls.

Conclusion: These results suggest that chitosan nanofibrous dressings enhance acute wound healing in rats by promoting re-epithelialization, neovasculogenesis, and maintaining low TNF-α levels in the later phases of healing.

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壳聚糖纳米纤维敷料促进大鼠急性创伤模型血管生成和抗炎反应的比较研究。

目的：壳聚糖基材料具有抗菌、生物相容性和生物降解性等优点，但其愈合机制尚不清楚。本研究探讨壳聚糖纳米纤维敷料伤口愈合的细胞和分子机制。方法：将39只Sprague Dawley大鼠按敷料类型分为3组：(1)壳聚糖纳米纤维敷料+透明膜组，(2)商业支架+透明膜组，(3)单独使用透明膜组（对照组）。在背侧创面全层创面（2cm × 2cm），用夹板固定，并用敷料覆盖。第7、14和21天的评估包括组织学分析，并测量伤口中TNF-α和iNOS的水平。结果：第21天，壳聚糖组的上皮化率明显高于支架组（87.5% vs. 42.0%, p = 0.03）。两组患者TNF-α水平均低于对照组。壳聚糖组CD68+/CD163+比值低于支架组（0.28 vs. 0.62, p = 0.037），血管形成高于对照组。结论：壳聚糖纳米纤维敷料可促进大鼠急性创面愈合，促进创面再上皮化、新生血管生成，并在愈合后期维持较低的TNF-α水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Biomedical Engineering 工程技术-工程：生物医学

CiteScore

7.50

自引率

15.80%

发文量

212

审稿时长

3 months

期刊介绍： Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.