Detection of PFAS and nanoplastics in serum by using Ag nanowires film SERS substrate with good reusability and sensitivity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-10-08 DOI:10.1007/s00604-025-07585-0

Yansheng Liu, Yukun Sun, Guofu Wang, Xiaobo Jia, Jin Zhou, Hongqi Li, Hongli Li, Haixin Chan, Kang Li, Zhicheng Jin, Zhaoxu Li

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引用次数: 0

Abstract

A novel surface-enhanced Raman spectroscopy (SERS)-based approach is introduced for the highly sensitive detection of perfluoroalkyl and polyfluoroalkyl substances (PFAS), polytetrafluoroethylene (PTFE), and polystyrene (PS) nanoplastics in serum samples using silver nanowire mesh (AgNWM) as SERS substrates. The surface-enhanced Raman scattering performance of AgNWM substrates was optimized using rhodamine 6G as a probe molecule, achieving a limit of detection (LOD) of 10⁻⁹ M. Serum samples were subjected to pre-treatment steps, including protein removal, sample enrichment, and concentration, enabling quantitative detection of multiple PFAS species at as low as 10⁻⁸ M. PTFE and PS nanoplastics were also successfully identified at a concentration of 100 ng·mL⁻¹. Furthermore, the AgNWM substrates exhibited significant stability and could be reused up to 10 times for plastic detection without substantial loss of sensitivity. This highly sensitive SERS platform provides an innovative and effective methodology for the detection of PFAS, PTFE, and PS nanoplastics in complex biological matrices, with considerable potential for clinical and environmental analyses.

Graphical Abstract

查看原文本刊更多论文

利用银纳米线膜SERS底物检测血清中PFAS和纳米塑料，具有良好的可重复使用性和灵敏度。

介绍了一种基于表面增强拉曼光谱（SERS）的新型方法，以银纳米线网（AgNWM）为底物，对血清样品中的全氟烷基和多氟烷基物质（PFAS）、聚四氟乙烯（PTFE）和聚苯乙烯（PS）纳米塑料进行高灵敏度检测。以罗丹明6G为探针分子，优化AgNWM底物的表面增强拉曼散射性能，达到10-9 m的检出限（LOD），血清样品经过预处理步骤，包括蛋白质去除、样品富集和浓缩，可以在低至10-8 m的浓度下定量检测多种PFAS， PTFE和PS纳米塑料也在100 ng·mL-1的浓度下成功鉴定。此外，AgNWM衬底表现出显著的稳定性，可以重复使用多达10次用于塑料检测，而不会显著降低灵敏度。这种高灵敏度的SERS平台为复杂生物基质中PFAS、PTFE和PS纳米塑料的检测提供了一种创新而有效的方法，具有相当大的临床和环境分析潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.