Indocyanine Green-Labeled Antibody-NOTCH2 as a New Fluorescent Molecular Imaging Probe for Gastric Cancer.

IF 3.9 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Bioconjugate Chemistry Pub Date : 2025-10-08 DOI:10.1021/acs.bioconjchem.5c00465

Mei Li, Jinglan Wang, Qinan Li, Xiaoxia Zhang, Luxi Yang, Yumin Li, Wenting He, Tao Liu

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引用次数: 0

Abstract

Gastric cancer is a malignant tumor that seriously threatens human health. Its high mortality is mainly due to delayed diagnosis, which makes early detection important for improving patient prognosis. Near-infrared (NIR) fluorescence imaging, with its high signal-to-noise ratio and good sensitivity, has been widely applied in biomedical research and clinical diagnosis. NOTCH2 is often overexpressed in gastric cancer and may serve as a useful target for diagnosis and treatment. In this study, we constructed a NOTCH2-targeted single-chain variable fragment (scFv) conjugated with indocyanine green (ICG) and evaluated its application in gastric cancer imaging in vitro and in vivo. The ICG-scFv probe showed similar photophysical properties to free ICG and was specifically taken up by MKN45 gastric cancer cells. In NIR imaging, ICG-scFv selectively accumulated in tumor tissue, achieved tumor-specific imaging, and maintained fluorescence signals for a longer time, which was further confirmed by colocalization analysis. These results indicate that the targeted fluorescent probe ICG-scFv may have potential value in the diagnosis and treatment of gastric cancer.

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吲哚菁绿色标记抗体notch2作为新型胃癌荧光分子成像探针

胃癌是一种严重威胁人类健康的恶性肿瘤。其高死亡率主要是由于诊断延迟，因此早期发现对于改善患者预后非常重要。近红外（NIR）荧光成像以其高信噪比和良好的灵敏度在生物医学研究和临床诊断中得到广泛应用。NOTCH2在胃癌中经常过表达，可能作为诊断和治疗的有用靶点。本研究构建了notch2靶向单链可变片段（scFv）结合吲哚菁绿（ICG），并对其在体外和体内胃癌成像中的应用进行了评价。ICG- scfv探针具有与游离ICG相似的光物理性质，并被MKN45胃癌细胞特异性吸收。在近红外成像中，ICG-scFv在肿瘤组织中选择性积累，实现肿瘤特异性成像，荧光信号维持时间较长，共定位分析进一步证实了这一点。这些结果表明靶向荧光探针ICG-scFv在胃癌的诊断和治疗中可能具有潜在的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioconjugate Chemistry 生物-化学综合

CiteScore

9.00

自引率

2.10%

发文量

236

审稿时长

1.4 months

期刊介绍： Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.