Asymmetric Janus Electrospun Nanofiber Wound Dressing Infused with Antibacterial and Anti-inflammatory Properties.

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-10-08 DOI:10.1021/acs.biomac.5c01166

Jing Zhao, Na Liu, Yating Zeng, Yiwen Zhang, Liyun Chen, Jianda Zhou, Bo Liu, Wancong Zhang, Shijie Tang

{"title":"Asymmetric Janus Electrospun Nanofiber Wound Dressing Infused with Antibacterial and Anti-inflammatory Properties.","authors":"Jing Zhao, Na Liu, Yating Zeng, Yiwen Zhang, Liyun Chen, Jianda Zhou, Bo Liu, Wancong Zhang, Shijie Tang","doi":"10.1021/acs.biomac.5c01166","DOIUrl":null,"url":null,"abstract":"Infection, sustained inflammation, and angiogenesis disorders delay skin wound healing. Consequently, there is a substantial demand for bioactive wound dressings that exhibit antibacterial properties, modulate inflammation, and facilitate wound healing. In this study, we developed a double-layer bioactive Janus electrospun nanofiber (ESF) dressing endowed with antibacterial and anti-inflammatory capabilities. The beaded hydrophobic outer layer acts as a physical barrier, preventing external fluids from contaminating the dressing and discouraging microbial attachment and growth. In contrast, the coaxial hydrophilic inner layer supports cell adhesion and migration. The results of the CCK-8 test and in vitro scratch wound healing assay of fibroblasts and endothelial cells demonstrated that the polycaprolactone/phycocyanin/chitosan/poly(ethylene oxide)/ε-poly-l-lysine Janus ESF dressing increased cell viability and migration. The RAW264.7 macrophage phenotypic switching assay results indicated the anti-inflammatory effects of the dressing. The dressing also exhibited a satisfactory antibacterial effect. Herein, the bioactive Janus ESF dressing hold considerable promise for wound healing.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.4000,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomacromolecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.biomac.5c01166","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Infection, sustained inflammation, and angiogenesis disorders delay skin wound healing. Consequently, there is a substantial demand for bioactive wound dressings that exhibit antibacterial properties, modulate inflammation, and facilitate wound healing. In this study, we developed a double-layer bioactive Janus electrospun nanofiber (ESF) dressing endowed with antibacterial and anti-inflammatory capabilities. The beaded hydrophobic outer layer acts as a physical barrier, preventing external fluids from contaminating the dressing and discouraging microbial attachment and growth. In contrast, the coaxial hydrophilic inner layer supports cell adhesion and migration. The results of the CCK-8 test and in vitro scratch wound healing assay of fibroblasts and endothelial cells demonstrated that the polycaprolactone/phycocyanin/chitosan/poly(ethylene oxide)/ε-poly-l-lysine Janus ESF dressing increased cell viability and migration. The RAW264.7 macrophage phenotypic switching assay results indicated the anti-inflammatory effects of the dressing. The dressing also exhibited a satisfactory antibacterial effect. Herein, the bioactive Janus ESF dressing hold considerable promise for wound healing.

查看原文本刊更多论文

非对称Janus静电纺纳米纤维伤口敷料注入抗菌和抗炎性能。

感染、持续炎症和血管生成障碍会延迟皮肤伤口愈合。因此，对具有抗菌性能、调节炎症和促进伤口愈合的生物活性伤口敷料的需求很大。在这项研究中，我们开发了一种具有抗菌和抗炎功能的双层生物活性Janus静电纺纳米纤维（ESF）敷料。珠状疏水外层充当物理屏障，防止外部流体污染敷料，阻止微生物附着和生长。相反，同轴亲水性内层支持细胞粘附和迁移。CCK-8实验及成纤维细胞和内皮细胞的体外划伤愈合实验结果表明，聚己内酯/藻蓝蛋白/壳聚糖/聚环氧乙烷/ε-聚赖氨酸Janus ESF敷料可提高细胞活力和迁移能力。RAW264.7巨噬细胞表型转换实验结果表明，该敷料具有抗炎作用。该敷料也表现出令人满意的抗菌效果。因此，具有生物活性的Janus ESF敷料在伤口愈合方面具有相当大的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.