Plant-specific BLISTER modulates miRNA biogenesis by regulating MIR transcription, HYL1 phosphorylation, and nuclear transport in Arabidopsis

IF 13.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleic Acids Research Pub Date : 2025-10-09 DOI:10.1093/nar/gkaf1012

Shu Wang, Xin Xin, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang

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引用次数: 0

Abstract

MicroRNAs (miRNAs), processed from primary transcripts by the microprocessor complex (MC), serve as crucial post-transcriptional regulators in eukaryotes. The stability and nuclear localization of HYL1, a core MC component, are essential for maintaining complex activity. In this study, we demonstrate that the plant-specific protein BLISTER (BLI) plays a key role in miRNA biogenesis by regulating MIR transcription, HYL1 phosphorylation, and HYL1 transport in Arabidopsis. The bli mutant exhibits increased accumulation of specific miRNAs accompanied by enhanced HYL1-containing D-body formation. Biochemical evidence indicates that BLI negatively regulates MIR transcription. Moreover, BLI promotes HYL1 dephosphorylation, which facilitates its degradation. Furthermore, BLI interacts with KETCH1 to orchestrate HYL1 nuclear import. These findings establish a novel regulatory paradigm where a plant-specific protein integrates transcriptional control and post-translational modification to coordinate miRNA production, advancing our understanding of plant gene regulation mechanisms.

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拟南芥植物特异性BLISTER通过调节MIR转录、HYL1磷酸化和核转运来调节miRNA的生物发生

微rna （miRNAs）是由微处理器复合物（MC）从初级转录本加工而成的，在真核生物中起着重要的转录后调节作用。mc1核心成分HYL1的稳定性和核定位对维持复合物活性至关重要。在这项研究中，我们证明了植物特异性蛋白BLISTER （BLI）在拟南芥中通过调节MIR转录、HYL1磷酸化和HYL1转运在miRNA生物发生中起关键作用。bli突变体表现出特异性mirna的积累增加，同时含有hyl1的d体形成增强。生化证据表明BLI负调控MIR转录。此外，BLI促进HYL1的去磷酸化，从而促进其降解。此外，BLI与KETCH1相互作用以协调HYL1核导入。这些发现建立了一种新的调控模式，其中植物特异性蛋白结合转录控制和翻译后修饰来协调miRNA的产生，促进了我们对植物基因调控机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nucleic Acids Research 生物-生化与分子生物学

CiteScore

27.10

自引率

4.70%

发文量

1057

审稿时长

2 months

期刊介绍： Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.