Peroxynitrite-responsive dicyanoisophorone-xanthene NIR fluorescent probe for monitoring and therapeutic evaluation of diabetic liver injury

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-10-01 DOI:10.1016/j.saa.2025.127003

Fei Kong , Ming Yang , Tao Yang , Jingcan Qin

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Abstract

Chronic hyperglycemia in diabetes induced liver injury (DLI) may progress to irreversible outcomes without timely intervention. It is highly beneficial to the development of a simple and rapid method for the early and accurate diagnosis of DLI. In this study, we developed an activatable near-infrared (NIR) fluorescent probe (DCIP-ONOO) based on a dicyanoisophorone-xanthene scaffold, for monitoring and therapeutic evaluation of DLI via Peroxynitrite (ONOO⁻) imaging. Upon the addition of ONOO⁻, DCIP-ONOO exhibited a 27.6-fold fluorescence enhancement at 770 nm with an excellent selectivity, fast responsiveness, and a low limit of detection (119.6 nmol/L). In vitro imaging experiments confirmed that it was capable of being employed for the sensitive detection of ONOO⁻. More significantly, in a diabetic mice model, DCIP-ONOO was successfully used to achieve in vivo imaging of DLI and to monitor metformin-mediated symptom alleviation without causing any organ damages. These results established DCIP-ONOO as a potential tool for effective tracking of ONOO⁻ during diabetic hepatotoxicity.

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过氧亚硝酸盐反应型二氰异硫酮-杂蒽近红外荧光探针对糖尿病性肝损伤的监测及疗效评价。

慢性高血糖在糖尿病引起的肝损伤（DLI）可能进展到不可逆的结果，没有及时的干预。为早期准确诊断DLI提供一种简便、快速的方法。在这项研究中，我们开发了一种可激活的近红外（NIR）荧光探针（DCIP-ONOO），基于二氰异膦酮-杂蒽支架，用于过氧亚硝酸盐（ONOO-）成像监测和治疗性评估DLI。添加ONOO-后，DCIP-ONOO在770 nm处荧光增强27.6倍，具有良好的选择性、快速的响应性和较低的检出限（119.6 nmol/L）。体外成像实验证实该方法可用于ONOO-的灵敏检测。更重要的是，在糖尿病小鼠模型中，DCIP-ONOO成功地实现了DLI的体内成像，并监测二甲双胍介导的症状缓解，而不造成任何器官损伤。这些结果确立了DCIP-ONOO作为有效追踪糖尿病肝毒性过程中ONOO-的潜在工具。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.