A magnetic SERS aptasensor integrated with Fe3O4@SiO2-Au nanocomposites for high-throughput, sensitive, and selective detection of sulfonamides

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-10-07 DOI:10.1007/s00604-025-07568-1

Jinxin Liu, Zhili Gao, Han Fu, Cheng Tian, Pengfei Shi, Shusheng Zhang

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引用次数: 0

Abstract

An innovative magnetic SERS aptasensor is presented that integrates Fe₃O₄@SiO₂-Au nanocomposites with aptamers, leveraging advancements in nanotechnology to overcome existing challenges. The aptasensor utilizes the magnetic Fe₃O₄ core for efficient separation from complex samples and the Au-decorated SiO₂ shell for signal enhancement via localized surface plasmon resonance. Moreover, aptamers with specificity for sulaquinoxaline (SQ), sulfamethoxypyridazine (SMP), and sulfamethoxydiazine (SMD) were used to enable selective recognition. Under optimized conditions, the detection limits of the aptasensor achieved were LOD_SQ = 33.26 pM, LOD_SMD = 63.11 pM, and LOD_SMP = 46.42 pM, with corresponding linear detection ranges of 0.1 nM to 50 μM, 0.1 nM to 50 μM, and 1 nM to 50 μM, respectively. It showed excellent selectivity against 11 interfering antibiotics and achieved recoveries of 91.4%–119.0% in real water samples (tap water, lake water, drinking water, seawater, effluent, river aquaculture water bodies, water bodies in lake poultry breeding areas, and milk). Moreover, the aptasensor demonstrates reliable performance in detecting sulfonamides in actual water samples, indicating its potential for applications in food safety and environmental monitoring.

Graphical Abstract

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一种集成Fe3O4@SiO2-Au纳米复合材料的磁性SERS适体传感器，用于高通量，敏感和选择性检测磺胺类药物。

提出了一种创新的磁性SERS适配体传感器，该传感器集成了Fe3O4@SiO2-Au纳米复合材料和适配体，利用纳米技术的进步来克服现有的挑战。该传感器利用磁性Fe3O4内核与复杂样品有效分离，并利用au修饰的SiO2外壳通过局部表面等离子体共振增强信号。此外，对磺胺喹啉（SQ）、磺胺甲氧基吡啶（SMP）和磺胺甲氧基吡啶（SMD）具有特异性的适配体可以进行选择性识别。在优化条件下，该传感器的检出限分别为LODSQ = 33.26 pM、LODSMD = 63.11 pM和LODSMP = 46.42 pM，线性检测范围分别为0.1 nM ~ 50 μM、0.1 nM ~ 50 μM和1 nM ~ 50 μM。对11种干扰抗生素具有良好的选择性，在自来水、湖水、饮用水、海水、污水、河流养殖水体、湖禽养殖区水体、牛奶等实际水样中回收率为91.4% ~ 119.0%。此外，该传感器在检测实际水样中的磺胺类物质方面表现出可靠的性能，表明其在食品安全和环境监测方面的应用潜力。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.