Total recovery of bacterioruberin from Arthrobacter via sustainable one-cycle extraction: A bench-scale guide to solvent selection and process intensification

IF 9 1区工程技术 Q1 ENGINEERING, CHEMICAL

Separation and Purification Technology Pub Date : 2025-10-08 DOI:10.1016/j.seppur.2025.135483

Angie V. Caicedo Paz, Daniele Giuffrida, Luigi Mondello, Alessia Tropea, Francesca Rigano, Fabiane O. Farias, Marcelo Franco, Cassamo U. Mussagy

{"title":"Total recovery of bacterioruberin from Arthrobacter via sustainable one-cycle extraction: A bench-scale guide to solvent selection and process intensification","authors":"Angie V. Caicedo Paz, Daniele Giuffrida, Luigi Mondello, Alessia Tropea, Francesca Rigano, Fabiane O. Farias, Marcelo Franco, Cassamo U. Mussagy","doi":"10.1016/j.seppur.2025.135483","DOIUrl":null,"url":null,"abstract":"Bacterioruberin (BR) is an uncommon C₅₀ natural carotenoid known for its exceptional antioxidant and antibacterial properties, making it highly promising for use in the food, pharmaceutical, and cosmeceutical sectors. Despite this potential, its commercial use is hindered by challenges in <em>downstream</em> processing. This research offers a comparative analysis of three methods to fully recover BR from <em>Arthrobacter</em> sp. CP30, a microorganism that produces approximately 95 % of BR and BR-derivatives. The first strategy, utilizing traditional methanol extraction, achieved a recovery rate of about 80 %, which could be improved to 100 % with optimized conditions. The second strategy employed binary solvent mixtures of alcohols and fatty acids, allowing for complete BR recovery under gentle extraction conditions. The third strategy combined green biosolvents, such as bio-based ethanol, with microwave-assisted extraction (MAE), resulting in a 100 % recovery rate and an Eco-Scale score of 89, indicating high environmental sustainability. In all methods, solvent selection was selected by COSMO-SAC modeling, which identified favorable molecular interactions between BR and the solvents tested. Overall, this work provides an efficient, sustainable, and scalable framework for BR recovery and serves as a practical guide for researchers and professionals to tailor extraction protocols according to their specific laboratory or industrial setups, to accomplish the full potential of high-value C₅₀ carotenoids.","PeriodicalId":427,"journal":{"name":"Separation and Purification Technology","volume":"26 1","pages":""},"PeriodicalIF":9.0000,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Separation and Purification Technology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.seppur.2025.135483","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Bacterioruberin (BR) is an uncommon C₅₀ natural carotenoid known for its exceptional antioxidant and antibacterial properties, making it highly promising for use in the food, pharmaceutical, and cosmeceutical sectors. Despite this potential, its commercial use is hindered by challenges in downstream processing. This research offers a comparative analysis of three methods to fully recover BR from Arthrobacter sp. CP30, a microorganism that produces approximately 95 % of BR and BR-derivatives. The first strategy, utilizing traditional methanol extraction, achieved a recovery rate of about 80 %, which could be improved to 100 % with optimized conditions. The second strategy employed binary solvent mixtures of alcohols and fatty acids, allowing for complete BR recovery under gentle extraction conditions. The third strategy combined green biosolvents, such as bio-based ethanol, with microwave-assisted extraction (MAE), resulting in a 100 % recovery rate and an Eco-Scale score of 89, indicating high environmental sustainability. In all methods, solvent selection was selected by COSMO-SAC modeling, which identified favorable molecular interactions between BR and the solvents tested. Overall, this work provides an efficient, sustainable, and scalable framework for BR recovery and serves as a practical guide for researchers and professionals to tailor extraction protocols according to their specific laboratory or industrial setups, to accomplish the full potential of high-value C₅₀ carotenoids.

查看原文本刊更多论文

通过可持续的单周期提取从节杆菌中完全回收细菌绿霉素：溶剂选择和工艺强化的实验规模指南

细菌胶（BR）是一种不常见的C₅0天然类胡萝卜素，以其卓越的抗氧化和抗菌性能而闻名，使其在食品，制药和药妆领域的应用前景广阔。尽管具有这种潜力，但其商业用途受到下游加工挑战的阻碍。本研究对从节肢细菌sp. CP30中完全回收BR的三种方法进行了比较分析，节肢细菌sp. CP30是一种产生大约95% % BR及其衍生物的微生物。第一种策略是利用传统的甲醇萃取，回收率约为80 %，优化条件后回收率可提高到100% %。第二种方法采用醇和脂肪酸的二元溶剂混合物，在温和的萃取条件下实现完全的BR回收。第三种策略将绿色生物溶剂（如生物基乙醇）与微波辅助萃取（MAE）相结合，回收率为100% %，生态尺度得分为89，表明环境可持续性高。在所有方法中，溶剂选择都是通过cosmos - sac模型选择的，该模型确定了BR与被测溶剂之间有利的分子相互作用。总体而言，这项工作为BR回收提供了一个高效，可持续和可扩展的框架，并作为研究人员和专业人员根据其特定实验室或工业设置定制提取方案的实用指南，以充分发挥高价值C₅0类胡萝卜素的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Separation and Purification Technology 工程技术-工程：化工

CiteScore

14.00

自引率

12.80%

发文量

2347

审稿时长

43 days

期刊介绍： Separation and Purification Technology is a premier journal committed to sharing innovative methods for separation and purification in chemical and environmental engineering, encompassing both homogeneous solutions and heterogeneous mixtures. Our scope includes the separation and/or purification of liquids, vapors, and gases, as well as carbon capture and separation techniques. However, it's important to note that methods solely intended for analytical purposes are not within the scope of the journal. Additionally, disciplines such as soil science, polymer science, and metallurgy fall outside the purview of Separation and Purification Technology. Join us in advancing the field of separation and purification methods for sustainable solutions in chemical and environmental engineering.