Generating theranostic bacteria by depositing a protective visual therapeutic nanocoating via in situ sequential mineralization

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-10-08 DOI:10.1126/sciadv.ady9183

Shixiong Chen, Beibei Sun, Lingling Lin, Xinxin Zhao, Yan Zhou, Feng Wu, Huilin Zhao, Jinyao Liu

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Abstract

Oral bacteriotherapy is increasingly exploited but faces challenges in simultaneously monitoring treatment process and achieving desirable efficacy. Here, we develop theranostic bacteria by depositing a protective visual therapeutic nanocoating via in situ sequential mineralization. Using two-step metal ion adsorption and nucleation, bacterial surface is successively mineralized with an inner manganese dioxide/ferric oxide nanoparticle layer and an outer calcium carbonate layer. Upon ingestion, the outer layer neutralizes gastric acid through double-decomposition reaction, protecting bacterial viability and the inner layer. During intestinal passage, the inner layer enables T1/T2 dual-mode magnetic resonance imaging for real-time tracking, whereas the lesion-triggered release of manganese ions allows T1 single-mode pathological detection. The inner layer also exhibits superoxide dismutase and catalase activities, synergizing therapeutic effects with bacteria. Further supported by satisfactory performances in two murine models of Salmonella-induced colitis and apolipoprotein E–deficient atherosclerosis, this work provides a platform to generate innovative theranostic agents for advanced bacteriotherapy.

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通过原位顺序矿化沉积保护性视觉治疗纳米涂层，产生治疗性细菌

口服细菌治疗越来越多地被利用，但在同时监测治疗过程和达到理想疗效方面面临挑战。在这里，我们通过原位顺序矿化沉积保护性视觉治疗纳米涂层来培养治疗性细菌。利用两步金属离子吸附和成核，细菌表面依次矿化，内层为二氧化锰/氧化铁纳米颗粒层，外层为碳酸钙层。摄入后，外层通过双重分解反应中和胃酸，保护细菌活力和内层。在肠道通道过程中，内层允许T1/T2双模磁共振成像实时跟踪，而病变触发的锰离子释放允许T1单模病理检测。内层也表现出超氧化物歧化酶和过氧化氢酶的活性，与细菌协同治疗作用。在沙门氏菌诱导的结肠炎和载脂蛋白e缺乏性动脉粥样硬化两种小鼠模型上的令人满意的表现进一步支持了这项工作，为产生先进细菌治疗的创新治疗药物提供了平台。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.