Salvia argentea L. extract inhibits the production of NO, and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), alleviates the inflammatory response of LPS-induced macrophages cells, and reduces the CRP level on carrageenan-induced paw edema.

IF 5.3 2区 医学 Q2 IMMUNOLOGY
Almonther Alhamedi, Tugce Demiroz Akbulut, Sura Baykan, Barış Gümüştaş, Ebru Sanci, Karrar Ali Mohammed Hasan Alsakini, Ayşe Nalbantsoy, Aylin Buhur, Altuğ Yavasoğlu, N Ülkü Karabay Yavasoğlu
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引用次数: 0

Abstract

Salvia argentea L. (Lamiaceae) is a medicinal plant originating from the Mediterranean region and has been used since ancient times for the treatment of various diseases. This study aimed to determine the phytochemical composition of S. argentea L. ethanol extract and to evaluate its in vitro and in vivo anti-inflammatory activity and its acute oral toxicity. The chemical constituents of the ethanol extract prepared from the aerial parts of the plant were identified using HPLC. The in vitro anti-inflammatory activity of the extract was evaluated in LPS-stimulated murine macrophage RAW 264.7 cells and the human monocytic cell line THP-1 by measuring the levels of nitric oxide (NO), pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). Acute toxicity of the extract was assessed in accordance with OECD guideline no 423. In vivo anti-inflammatory activity was evaluated based on the inhibition of 1% carrageenan-induced paw edema in rats. Serum CRP levels as an inflammatory marker, were measured via ELISA. Histological and immunohistochemical assessments were performed to identify tissue changes in the paw. HPLC profiling revealed that the extract contained rosmarinic acid (11.334 µg/mg dry extract), and salvigenin (2.74 µg/mg of dry extract) as major compounds. The extract significantly inhibited the production of NO, IL-1β, IL-6, and TNF-α without affecting cell viability. In vivo, the extract treatment exhibited a dose-dependent reduction in paw edema and serum CRP levels, along with notable histological improvements. Administration of the extract resulted in dose-dependent decreases of NF-κB expressions in the paw tissues. No signs of acute toxicity were observed (oral LD₅₀ > 2000 mg/kg). These findings suggest that S. argentea L. ethanol extract possesses significant anti-inflammatory potential supporting its possible development as a natural therapeutic agent for inflammatory disorders.

丹参提取物可抑制NO、促炎因子(IL-1β、IL-6、TNF-α)的产生,减轻脂多糖诱导的巨噬细胞的炎症反应,降低角叉菜胶诱导的足跖水肿CRP水平。
鼠尾草(Salvia argentea L.)是一种原产于地中海地区的药用植物,自古以来就用于治疗各种疾病。本研究旨在测定银青茶乙醇提取物的植物化学成分,并评价其体外和体内抗炎活性及其急性口服毒性。采用高效液相色谱法对该植物地上部分乙醇提取物的化学成分进行了鉴定。通过测定一氧化氮(NO)、促炎细胞因子(IL-1β、IL-6、TNF-α)水平,对lps刺激小鼠巨噬细胞RAW 264.7细胞和人单核细胞THP-1的体外抗炎活性进行评价。根据OECD第423号指南对提取物的急性毒性进行了评估。通过1%角叉菜胶对大鼠足跖水肿的抑制作用,评估其体内抗炎活性。血清CRP水平作为炎症标志物,通过ELISA检测。进行组织学和免疫组织化学评估以确定足部的组织变化。HPLC图谱显示,该提取物主要成分为迷迭香酸(11.334µg/mg干提取物)和丹参素(2.74µg/mg干提取物)。该提取物显著抑制NO、IL-1β、IL-6和TNF-α的产生,但不影响细胞活力。在体内,提取物治疗表现出剂量依赖性减少足跖水肿和血清CRP水平,以及显著的组织学改善。给药后,足跖组织中NF-κB表达呈剂量依赖性降低。未观察到急性毒性迹象(口服LD₅0 > 2000 mg/kg)。这些研究结果表明,银青茶乙醇提取物具有显著的抗炎潜力,支持其作为炎性疾病的天然治疗剂的可能发展。
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来源期刊
Inflammopharmacology
Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY
CiteScore
8.00
自引率
3.40%
发文量
200
期刊介绍: Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]
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