Seasonal and COVID-19 lockdown variations in PM_2.5 composition to apoptosis pathways and chemical toxicity in lung cells.

IF 2.2 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. General subjects Pub Date : 2025-10-04 DOI:10.1016/j.bbagen.2025.130864

Ting-Hsuan Wu, Yu-Cheng Chen, Hong-Lin Chan, Hsiu-Chuan Chou

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引用次数: 0

Abstract

Objective: To investigate seasonal and COVID-19-lockdown variations in PM2.5 chemical composition in Taipei and the effects of those compositional differences on lung cell toxicity.

Methods: PM2.5 was collected in warm (2021/07-2021/08) and cold (2021/11-2022/02) seasons and during LV2/LV3 COVID-19 alert periods. Composition (metals, PAHs) was analyzed and A549 and PC9 lung cells were exposed to equal mass concentrations (0-100 μg/mL) of extracted PM2.5. Cell viability, ROS, apoptosis, mitochondrial membrane potential and GSH/GPX4 were measured.

Results: At equivalent mass concentrations (50 μg/mL), warm-season PM2.5 induced higher ROS and greater reduction in viability than cold-season PM2.5. LV2-period PM2.5 contained higher metal content and caused more severe cellular damage than LV3. Spearman correlation plus single-compound assays identified cadmium and Dibenzo[a,e]pyrene as components strongly associated with cytotoxicity.

Conclusions: Seasonal and human-activity driven compositional changes-rather than mass concentration alone-affect PM2.5 toxicity in vitro; these results underscore the importance of controlling specific chemical emissions.

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PM2.5成分对肺细胞凋亡途径和化学毒性的季节性和COVID-19封锁变化。

目的：探讨台北市PM2.5化学成分的季节性变化及其对肺细胞毒性的影响。方法：在温暖季节（2021/07-2021/08）和寒冷季节（2021/11-2022/02）以及LV2/LV3 COVID-19预警期采集PM2.5。分析其组成（金属、多环芳烃），并将A549和PC9肺细胞暴露于等质量浓度（0-100 μg/mL）的提取PM2.5中。测定细胞活力、ROS、凋亡、线粒体膜电位和GSH/GPX4。结果：在同等质量浓度（50 μg/mL）下，暖季PM2.5比冷季PM2.5诱导更高的ROS和更大的活力降低。与LV3相比，lv2期PM2.5的金属含量更高，造成的细胞损伤更严重。Spearman相关性加单化合物测定鉴定出镉和二苯并[a，e]芘是与细胞毒性密切相关的成分。结论：季节性和人类活动驱动的成分变化，而不是质量浓度单独影响PM2.5的体外毒性；这些结果强调了控制特定化学物质排放的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochimica et biophysica acta. General subjects 生物-生化与分子生物学

CiteScore

6.40

自引率

0.00%

发文量

139

审稿时长

30 days

期刊介绍： BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.