Stingless Bee Honey as a Therapeutic Strategy: Enhancing Neuroprotective and Cognitive Function in a Seizure Model Induced by Kainic Acid.

IF 2.3 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2025-10-04 DOI:10.1016/j.bbr.2025.115857

Nurdarina Ausi Zulkifli, Anani Aila Mat Zin, Zurina Hassan, Zulkifli Mustafa, Wan Norlina Wan Azman, Siti Nurma Hanim Hadie, Nurhafizah Ghani

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引用次数: 0

Abstract

Kainic acid (KA) has been widely used in studies due to its ability to mimic pathologic traits and comorbidities in human epilepsy. Studies have reported that stingless bee honey (SBH) may have neuroprotective effects by mitigating neuronal cell death and the inflammation pathway. Therefore, this study aims to evaluate SBH's neuroprotective effects on a seizure model induced by KA. The male Sprague Dawley rats were randomly divided into six groups (n=6/groups): control, SBH (4.6g/kg), SBH (9.3g/kg), KA, KA+SBH (4.6g/kg) and KA+SBH (9.3g/kg). The rats were induced by KA with repeated low doses (5mg/kg) at 30-minute intervals. Rats undergo Video-Electroencephalogram (V-EEG) assessment at 7-day intervals and daily treatment for 28 days. Morris water maze (MWM), cresyl-violet and Fluoro-Jade C (FJC) were evaluated in all groups. SBH (4.6g/kg) showed significantly less time of escape latency than the KA group (p = 0.0088). A greater number of platforms crossing was observed in the SBH (4.6g/kg) than the KA group (p=0.0240). The SBH-treated KA-induced group had significantly increased viable cells than KA only (p=0.0072, p<0.001). SBH's strong antioxidants and anti-inflammatory properties might contribute to its neuroprotective effects, thus promoting neuronal survival in a seizure model induced by KA.

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无刺蜂蜜作为一种治疗策略：在Kainic酸诱导的癫痫模型中增强神经保护和认知功能。

Kainic acid （KA）由于其能够模拟人类癫痫的病理特征和合并症而被广泛应用于研究。研究报道，无刺蜂蜜（SBH）可能通过减轻神经元细胞死亡和炎症途径具有神经保护作用。因此，本研究旨在评估SBH对KA诱导的癫痫发作模型的神经保护作用。雄性sd大鼠随机分为对照组、SBH组（4.6g/kg）、SBH组（9.3g/kg）、KA组、KA+SBH组（4.6g/kg）、KA+SBH组（9.3g/kg） 6组（n=6/组）。以低剂量（5mg/kg） KA诱导大鼠，每隔30分钟重复一次。大鼠每隔7天进行一次视频脑电图（V-EEG）评估，每天给药28天。各组采用Morris水迷宫（MWM）、甲酚紫（creyl -violet）和氟玉C （Fluoro-Jade C， FJC）进行评价。SBH （4.6g/kg）组的逃避潜伏期明显少于KA组（p = 0.0088）。与KA组相比，SBH组（4.6g/kg）的平台穿越次数更多（p=0.0240）。sbh处理KA诱导组的活细胞明显高于KA单独处理组(p=0.0072, p

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.