Investigation of Anti-Toxoplasma gondii Antibodies and Molecular Analysis in Nonhuman Primates Under Human Care in Rio de Janeiro, Brazil

IF 0.5 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Medical Primatology Pub Date : 2025-10-05 DOI:10.1111/jmp.70032

Natalie Renata Zorzi, Júlia Trevizan Segalin, Cláudia Dazzi, Franciele Oliveira, Andressa Ferreira da Silva, Leila Maria Carvalho Alves, Anna Elisa Athayde-Gusmão, Olivia Zen Gianfrancisco, Daniel Almeida Balthazar, Fernanda Correa Rocha, Clarissa Vieira Lopes, Bruna Azevedo Baêta, Matheus Dias Cordeiro, Silvia Bahadian Moreira, Alcides Pissinatti, Daniel Guimarães Ubiali, Márcio Machado Costa, Maria Isabel Botelho Vieira

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Abstract

Introduction

This study aimed to conduct a serological and molecular survey of Toxoplasma gondii in nonhuman primates under human care in the state of Rio de Janeiro.

Materials and Methods

MAT and qPCR were performed on 199 serum and blood samples collected from animals at the following locations: BioParque Zoo, Volta Redonda Municipal Zoo, Wildlife Rehabilitation Center, Laboratory Animal Facility of the Federal University of Rio de Janeiro, and the Rio de Janeiro Primatology Center. Risk factors for T. gondii infection were also assessed.

Results

qPCR analysis revealed DNA amplification of T. gondii in one animal of the species Alouatta caraya. MAT analysis showed 100% seropositivity among Old World primates and 42.62% among New World primates.

Conclusion

At locations where raw meat is included in the diet and water is unfiltered, animals were found to be 10.88 times more likely to test seropositive.

Abstract Image

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巴西巴西巴西里约热内卢de Janeiro人类照料下非人灵长类动物抗刚地弓形虫抗体调查及分子分析。

前言：本研究旨在对巴西里约热内卢州人类照料下非人灵长类动物的刚地弓形虫进行血清学和分子调查。材料和方法：采用MAT和qPCR对下列地点的199份动物血清和血液样本进行分析：BioParque动物园、Volta Redonda市立动物园、野生动物康复中心、巴西里约热内卢联邦大学实验动物设施和巴西里约热内卢灵长类学中心。还评估了弓形虫感染的危险因素。结果：qPCR分析结果显示，1只阿鲁阿塔卡拉亚动物体内有弓形虫DNA扩增。MAT分析显示，旧大陆灵长类动物血清阳性率为100%，新大陆灵长类动物血清阳性率为42.62%。结论：在饮食中含有生肉且水未经过滤的地方，动物血清检测呈阳性的可能性高出10.88倍。

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.