A novel approach to poliovirus vaccine neurovirulence testing: development of an enhanced 4-level scoring system

IF 2.3 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2025-09-29 DOI:10.1016/j.imbio.2025.153121

Xi Wang , Yunguang Hu , Anguo Yin , Changxu Chen , Hong Xiang , Li Wang , Bochuan Liu , Yuan Tian , Yu Gao , Mingrun Zhang , Yan Li , Yina Cun , Jian Zhou

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Abstract

The ongoing promotion and administration of vaccines play a critical role in the global effort to eradicate poliovirus. The Monkey Neurovirulence Test (MNVT) is an essential method for evaluating the neurovirulence of viruses in both the Oral Attenuated Live Poliovirus Vaccine (OPV) and the Sabin Inactivated Poliovirus Vaccine (sIPV) to ensure they meet required safety standards. The “4-level scoring method” recommended by the World Health Organization (WHO) has served as a key pathological evaluation criterion in this test. However, its lack of scoring granularity often leads to subjective interpretation discrepancies among evaluators, which compromises the consistency and reliability of the MNVT results. To address these limitations, we have mapped the primary neuronal nuclei in monkey tissue sections and analyzed viral-induced damage distribution. As a result, we developed an Enhanced 4-Level Scoring System (integrating precise anatomical mapping and quantitative lesion analysis), which minimizes evaluator bias and significantly improves scoring stability and consistency.

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脊髓灰质炎疫苗神经毒力检测的新方法：增强型4级评分系统的发展。

正在进行的疫苗推广和接种工作在全球根除脊髓灰质炎病毒的努力中发挥着关键作用。猴神经毒力试验（MNVT）是评估口服脊髓灰质炎减毒活疫苗（OPV）和沙宾灭活脊髓灰质炎疫苗（sIPV）中病毒神经毒力的重要方法，以确保它们符合规定的安全标准。世界卫生组织（WHO）推荐的“4级评分法”作为本试验的主要病理评价标准。然而，它缺乏评分粒度，常常导致评估者之间的主观解释差异，从而损害了MNVT结果的一致性和可靠性。为了解决这些局限性，我们绘制了猴组织切片的初级神经元核，并分析了病毒诱导的损伤分布。因此，我们开发了一个增强的4级评分系统（整合精确的解剖制图和定量的病变分析），它最大限度地减少了评估者的偏见，并显著提高了评分的稳定性和一致性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.