Authors' response to letter to the editor: Sex differences in neuropathological response to traumatic brain injury: increased neuronal loss and astrogliosis in females.

Abstract

The present correspondence constitutes a response to the Letter to the Editor regarding our publication Sex Differences in Neuropathological Response to Traumatic Brain Injury: Increased Neuronal Loss and Astrogliosis in Females. We are pleased that our paper has attracted attention and hereby provide comments on the authors' observations and suggestions. In this reply, we clarify our rationale for analysing the cerebral cortex, the absence of behavioural data, and the choice of the 30-day post-injury timeframe, which was based on prior experience with this model of brain injury. Furthermore, we include information on the evaluation of the oestrous cycle as well as details of the image processing applied during the morphological analysis of glial cells. Finally, we outline our plans to broaden the scope of the study through the investigation of an alternative therapeutic approach to traumatic brain injury, incorporating multiple in vitro and in vivo methodologies, including histological, biochemical, and imaging techniques.