Enzymatic and biosurfactant production with antimicrobial activity by Bacillus subtilis group.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-10-06 DOI:10.1007/s00203-025-04486-x

Mariana Prósperi de Oliveira Paula, Clara Resende de Souza Castro, Juliete Gomes de Lara, Luciana Silva Ribeiro, Victor Satler Pylro, Cristina Ferreira Silva

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引用次数: 0

Abstract

Biosurfactants are valuable microbial metabolites with broad industrial and environmental applications. In this study, we investigated biosurfactant production by Bacillus subtilis group strains using residual glycerol as the sole carbon source, while also evaluating their enzymatic activity and antimicrobial properties. PCR-based gene detection confirmed the presence of genes encoding surfactin, fengycin, and iturin peptide biosynthesis. Crude glycerol, a byproduct of biodiesel production, supported biosurfactant synthesis by the tested strains. Strains CCMA 0087, 0658, and 2031 exhibited diesel oil emulsification rates ranging from approximately 53 to 61%, while also reducing water surface tension to around 30 mN/m. Notably, the crude biosurfactant produced by Bacillus velezensis CCMA 0087 inhibited Escherichia coli growth, whereas Bacillus subtilis CCMA 2031 partially inhibited Fusarium paranaense (~ 38%), representing the first report of biosurfactant activity against this phytopathogen. Ligninolytic enzyme assays revealed that strain 2031 exhibited the highest manganese peroxidase (MnP) activity, while 0087 showed the greatest lignin peroxidase (LiP) activity. These findings highlight the potential of biosurfactants and ligninolytic enzymes from Bacillus subtilis group strains for biocontrol and bioremediation applications.

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枯草芽孢杆菌群生产具有抗菌活性的酶和生物表面活性剂。

生物表面活性剂是一种有价值的微生物代谢物，具有广泛的工业和环境应用。在这项研究中，我们研究了枯草芽孢杆菌群菌株以残余甘油为唯一碳源生产生物表面活性剂，同时评估了它们的酶活性和抗菌性能。基于pcr的基因检测证实存在编码表面蛋白、风霉素和iturin肽生物合成的基因。粗甘油是生物柴油生产的副产物，支持被试菌株合成生物表面活性剂。菌株CCMA 0087、0658和2031的柴油乳化率约为53%至61%，同时也将水的表面张力降低到30 mN/m左右。值得注意的是，velezensis芽孢杆菌CCMA 0087产生的粗生生物表面活性剂对大肠杆菌的生长有抑制作用，而枯草芽孢杆菌CCMA 2031产生的粗生生物表面活性剂对副镰刀菌的生长有部分抑制作用（~ 38%），这是生物表面活性剂对该植物病原体活性的首次报道。木质素分解酶测定结果显示，菌株2031的锰过氧化物酶（MnP）活性最高，菌株0087的木质素过氧化物酶（LiP）活性最高。这些发现突出了枯草芽孢杆菌群菌株的生物表面活性剂和木质素降解酶在生物防治和生物修复方面的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.