High-Performance Sn/AuNP-Based Spectroscopic Ellipsometric Sensor for Patulin.

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-10-03 DOI:10.1016/j.ab.2025.115987

Zafer Üstündağ, Sinem Tunçer Çağlayan, Mustafa Oguzhan Caglayan

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Abstract

A label-free spectroscopic ellipsometric (SE) aptasensor was developed for ultra-sensitive patulin (PAT) detection on a tin (Sn) substrate modified via electrochemical grafting of a 4-mercaptobenzene diazonium-derived self-assembled monolayer. Cyclic voltammetry and high-resolution X-ray photoelectron spectroscopy confirmed uniform nanofilm formation, followed by citrate-reduced gold nanoparticle decoration and thiolated anti-PAT aptamer immobilization. Monitoring the ellipsometric phase shift (Δ) at 507 nm and 508 nm yielded linear responses over 0.01-1000 ng/mL PAT (R² > 0.98) with limits of detection of 10.7 pg/mL and 9.5 pg/mL (3.3 σ), respectively. Isotherm fitting indicated pseudo-second-order binding (R² = 1.00), consistent with surface-constrained, mass-transfer-limited interactions. Precision (%RSD < 1.1 %) and accuracy (%RE within ±5 %) were validated in both buffer and spiked apple-juice samples (recoveries 98.9-103.2 %). This label-free SE approach combines a wide dynamic range and sub-10 pg/mL sensitivity with minimal sample preparation and no external labels.

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基于Sn/ aunp的展青霉素光谱椭偏传感器。

在电化学接枝4-巯基苯重氮衍生自组装单层修饰的锡（Sn）衬底上，研制了一种用于超灵敏展青霉素（PAT）检测的无标记光谱椭偏（SE）适体传感器。循环伏安法和高分辨率x射线光电子能谱法证实了均匀的纳米膜形成，随后是柠檬酸还原金纳米颗粒装饰和硫代抗pat适配体固定。在507 nm和508 nm处监测椭偏相移（Δ），在0.01 ~ 1000 ng/mL PAT （R2 > 0.98）范围内产生线性响应，检出限分别为10.7 pg/mL和9.5 pg/mL （3.3 σ）。等温线拟合显示伪二阶结合（R2 = 1.00），符合表面约束的、传质受限的相互作用。精密度（%RSD < 1.1%）和准确度（%RE≤±5%）在缓冲液和加标苹果汁样品中均得到验证（回收率为98.9 ~ 103.2%）。这种无标签的SE方法结合了宽动态范围和低于10 pg/mL的灵敏度，最小的样品制备和无外部标签。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.