Improved hydrolyzed polyacrylamide biodegradation and gel breaking performance of composite bacteria

IF 3.2 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2025-10-05 DOI:10.1007/s10532-025-10196-4

Jiqian Wang, Xuanyu Zhang, Han Han, Yuqin Jia, Hai Zhang, Yanhui Jia, Hui Li

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引用次数: 0

Abstract

Polymer flooding technology enhances crude oil recovery but generates a large amount of wastewater containing hydrolyzed polyacrylamide (HPAM) and HPAM residue in oil reservoirs, which induce serious environmental problems. Effective degradation of HPAM is highly required in oilfields, especially biodegradation technologies. Ten strains of HPAM degrading bacteria have been screened and identified from oilfield wastewater. An optimal HPAM biodegradation system of composite bacteria has been established based on two strains Agrobacterium pusense NMYGYA2 and Stutzerimonas balearica SCE1. The HPAM biodegradation performance of the composite bacteria has been improved through cultivation condition optimization. The highest HPAM removal rate of 81.2% could be achieved at the optimized condition with the addition of 800 mg·L⁻¹ urea, 500 mg·L⁻¹ glucose and 50 mg·L⁻¹ CaCl₂. The gel permeation chromatography results showed that the HPAM molecular weight decreased from 3.7 × 10⁶ Da to 1.9 × 10⁵ Da after the composite bacterial degradation. Fourier transform infrared spectroscopy analysis revealed the hydrolysis of NH₂ group and the cleavage of C–C bond. Furthermore, the composite bacteria exhibited the ability to break down HPAM gels via biodegradation at temperatures up to 55 °C, indicating that they can be used to treat the blocking in reservoirs with a temperature < 55 °C and ground facilities.

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提高了复合菌对水解聚丙烯酰胺的生物降解和破胶性能。

聚合物驱技术提高了原油采收率，但在油藏中产生大量含水解聚丙烯酰胺（HPAM）的废水和HPAM残渣，造成了严重的环境问题。油田对HPAM的有效降解要求很高，尤其是生物降解技术。从油田废水中筛选鉴定了10株HPAM降解菌。以pusense农杆菌NMYGYA2和balearica Stutzerimonas SCE1两株菌株为研究对象，建立了复合菌对HPAM的最佳生物降解体系。通过优化培养条件，提高复合菌对HPAM的生物降解性能。在最佳条件下，尿素800 mg·L-1、葡萄糖500 mg·L-1、CaCl2 50 mg·L-1， HPAM去除率最高，达81.2%。凝胶渗透层析结果表明，复合菌降解后的HPAM分子量由3.7 × 106 Da降至1.9 × 105 Da。傅里叶红外光谱分析显示NH2基团水解，C-C键断裂。此外，复合细菌表现出在高达55°C的温度下通过生物降解分解HPAM凝胶的能力，这表明它们可以用于处理具有温度的储层堵塞

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.