First report of molecular characterization of Metastrongylus salmi in pigs from Karnataka, India using mitochondrial COX1 gene.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-11-01 Epub Date: 2025-10-03 DOI:10.1007/s13205-025-04534-9

Siju Susan Jacob, Snigdha Madhaba Maharana, Kempashi Jayachandra, Narayanaswamy Pradeep, Jagadish Hiremath, Sharanagouda S Patil, Pinaki Prasad Sengupta

{"title":"First report of molecular characterization of Metastrongylus salmi in pigs from Karnataka, India using mitochondrial COX1 gene.","authors":"Siju Susan Jacob, Snigdha Madhaba Maharana, Kempashi Jayachandra, Narayanaswamy Pradeep, Jagadish Hiremath, Sharanagouda S Patil, Pinaki Prasad Sengupta","doi":"10.1007/s13205-025-04534-9","DOIUrl":null,"url":null,"abstract":"The microscopical examination revealed the typical features of Metastrongylus salmi, in the present study. The PCR amplification and sequencing of the COX1 gene confirmed 99.7-100% nucleotide identity with reference to M. salmi sequences and less identity with other Metastrongylus species. The phylogenetic analysis grouped the Indian isolates with Brazilian M. salmi sequences in a well-supported clade. Pairwise identity analysis demonstrated clear genetic divergence from M. apri and M. pudendotectus. Haplotype network analysis revealed 15 distinct haplotypes across Metastrongylus species, indicating substantial genetic differentiation. Mismatch distribution analysis suggested a stable population of M. salmi with potential sub-structuring. These findings enhance our understanding of the genetic diversity and evolutionary patterns of M. salmi, with implications for its epidemiology and control.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04534-9.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 11","pages":"368"},"PeriodicalIF":2.9000,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494507/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"3 Biotech","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13205-025-04534-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/10/3 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The microscopical examination revealed the typical features of Metastrongylus salmi, in the present study. The PCR amplification and sequencing of the COX1 gene confirmed 99.7-100% nucleotide identity with reference to M. salmi sequences and less identity with other Metastrongylus species. The phylogenetic analysis grouped the Indian isolates with Brazilian M. salmi sequences in a well-supported clade. Pairwise identity analysis demonstrated clear genetic divergence from M. apri and M. pudendotectus. Haplotype network analysis revealed 15 distinct haplotypes across Metastrongylus species, indicating substantial genetic differentiation. Mismatch distribution analysis suggested a stable population of M. salmi with potential sub-structuring. These findings enhance our understanding of the genetic diversity and evolutionary patterns of M. salmi, with implications for its epidemiology and control.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04534-9.

查看原文本刊更多论文

利用线粒体COX1基因对印度卡纳塔克邦猪腊肠转圆线虫进行分子鉴定的首次报道。

显微镜检查显示了salmi后圆线虫的典型特征。PCR扩增和测序结果表明，COX1基因与M. salmi序列的核苷酸同源性为99.7-100%，与其他mettastrongylus物种的同源性较低。系统发育分析将印度分离株与巴西M. salmi序列归为一个支持良好的分支。两两同一性分析表明，apri和pudendotectus具有明显的遗传差异。单倍型网络分析显示，根线虫种间有15种不同的单倍型，表明存在显著的遗传分化。失配分布分析表明，这是一个稳定的种群，存在潜在的亚结构。这些发现增强了我们对米氏杆菌遗传多样性和进化模式的理解，对其流行病学和控制具有重要意义。补充信息：在线版本包含补充资料，下载地址：10.1007/s13205-025-04534-9。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.