Microbial degradation of diesel fuel as a potential environmental bioremediation

IF 3.2 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2025-10-04 DOI:10.1007/s10532-025-10194-6

Letícia Beatriz Ueda Melo, Bruna Bacaro Borrego, Louise Hase Gracioso, Elen Aquino Perpetuo

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Abstract

Diesel oil is a fossil fuel widely utilized globally and a significant source of environmental contamination. Its presence poses substantial ecological challenges. Bioremediation emerges as a viable solution for restoring diesel-contaminated environments, contingent upon comprehending the local microbiota. This study employed a metabarcoding technique with a culture-dependent approach to assess the impact of hydrocarbon contamination on soil microbiota. Soil samples were collected from contaminated areas, and microbial diversity was assessed through relative abundance, alpha, and beta diversity analyses. Additionally, bacterial strains isolated from the same area were screened for their ability to degrade hydrocarbons. Four strains, Pseudomonas nitroreducens B32, Pseudomonas koreensis B11, Ralstonia sp. BC2, and Acinetobacter sp. BC3 could degrade up to 38% of the diesel, using it as the sole carbon source. These strains effectively degraded n-alkanes and cyclic alkanes with short and medium chains (C7 to C18). This research enhances the understanding of hydrocarbons’ impacts on soil microbiota and underscores the potential application of microorganisms in bioremediation efforts.

Graphical abstract

Abstract Image

查看原文本刊更多论文

微生物降解柴油作为一种潜在的环境生物修复技术。

柴油是全球广泛使用的化石燃料，也是环境污染的重要来源。它的存在带来了巨大的生态挑战。生物修复是恢复柴油污染环境的一种可行的解决方案，这取决于对当地微生物群的了解。本研究采用元条形码技术和培养依赖方法来评估碳氢化合物污染对土壤微生物群的影响。从污染地区采集土壤样本，通过相对丰度、α和β多样性分析评估微生物多样性。此外，从同一地区分离的细菌菌株进行了筛选，以确定其降解碳氢化合物的能力。以柴油为唯一碳源的4种菌株，硝基还原假单胞菌B32、韩国假单胞菌B11、Ralstonia sp. BC2、Acinetobacter sp. BC3对柴油的降解率可达38%。这些菌株能有效降解短链和中链（C7 ~ C18）的正构烷烃和环构烷烃。该研究提高了对碳氢化合物对土壤微生物群影响的认识，并强调了微生物在生物修复工作中的潜在应用。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.