Analysis of blood components and metabolites of Gancao-Ganjiang decoction before and after compatibility based on UPLC-LTQ-Orbitrap-MS technique

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-10-02 DOI:10.1016/j.fitote.2025.106901

Kun Liang , Qianyun Zhang , Wenmin Dong , Wenxia Zhu , Xinhong Wang , Rui An , Lisha You

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引用次数: 0

Abstract

Objective

To study the effect of compatibility of Gancao-Ganjiang Decoction on difference in serum components and metabolites of normal rats.

Methods

Ultrahigh performance liquid chromatography combined linear ion trap electrostatic field orbitrap mass spectrometry (UPLC-LTQ Orbitrap MS) was used to detect the drug containing serum before and after the compatibility of Glycyrrhizae Radix Et Rhizoma (GanCao,GC) and Zingiberis Rhizoma (Ganjiang,GJ), and the prototype components identified were detected and analyzed with Xcalibur 2.2.0. Compound Discover 3.3 software was applied to speculate the possible metabolites and their pathways.

Results

A total of 23 prototype components were identified before and after the compatibility of Gancao-Ganjiang Decoction, 20 metabolites were identified in the pre-compatibility group, and 18 metabolites were identified in the post compatibility group. According to the metabolic pathway, ion fragment information and software technology, it was speculated that the metabolic pathways before compatibility were mainly the reducing of glycine conjugation, increasing of glucuronidation, methylation and metoxylation reaction. Glycine conjugation, sulfation were increased after compatibility.

Conclusion

In normal rats, there is a significant difference in the metabolites before and after compatibility of Gancao-Ganjiang Decoction. After compatibility, the prototype of the main active ingredients and their metabolite pathways are more widely distributed, which further lays a foundation for the study of the compatibility of Gancao-Ganjiang Decoction, and also provides a scientific basis for clarifying the compatibility mechanism.

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基于UPLC-LTQ-Orbitrap-MS技术的甘草甘姜汤配伍前后血液成分及代谢物分析

目的：研究甘草甘姜汤配伍对正常大鼠血清成分及代谢物差异的影响。方法：采用超高效液相色谱联用线性离子阱静电场轨道阱质谱法（UPLC-LTQ orbitrap MS）对甘草根（GC）和姜根（赣江，GJ）配色前后含药血清进行检测，并对鉴定出的原型成分使用Xcalibur 2.2.0进行检测分析。应用Compound Discover 3.3软件推测可能的代谢物及其途径。结果：甘草-甘姜汤配伍前后共鉴定出23个原型组分，配伍前组鉴定出20个代谢物，配伍后组鉴定出18个代谢物。根据代谢途径、离子片段信息和软件技术推测，配伍前的代谢途径主要是甘氨酸偶联的减少，葡萄糖醛酸化、甲基化和甲氧基化反应的增加。配伍后甘氨酸偶联、磺化作用增强。结论：正常大鼠肝草甘姜汤配伍前后代谢产物有显著差异。配型后，主要有效成分原型及其代谢途径分布更为广泛，进一步为甘草-甘姜汤配型研究奠定了基础，也为阐明配型机制提供了科学依据。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.