Exploring the bioactivity potential of fruit-associated fungi: Antifungal, antiparasitic, and antiviral activities of natural extracts

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-10-02 DOI:10.1016/j.fitote.2025.106917

Vitor de Souza Mazucato , Ludmilla Tonani , Marcia Regina von Zeska Kress , Adriano Defini Andricopulo , Gisele Barbosa , Renata Krogh , Leonardo Luiz Gomes Ferreira , Natasha Marques Cassani , Bruna Coelho Sandim , Ana Carolina Gomes Jardim , Paulo Cezar Vieira

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Abstract

This study investigated the potential of fungi associated with protease-rich fruits, such as papaya and pineapple, as sources of bioactive extracts. Fungal strains were cultivated in different media (rice, corn, wheat, oats, and sugarcane bagasse. The resulting extracts were analyzed by ¹H NMR for chemical profiling, which allowed the observation of compounds such as rhizosolaniol, haematocin, beauvericin, cyclosporines, and fusaridioic acid. The extracts cultivated on rice exhibited high papain inhibitory activity, particularly those from Neofusicoccum ribis (99.4 % inhibition at 50 μg/mL) and Fusarium sacchari (89.6 % at 50 μg/mL). Additionally, promising anti-Trypanosoma (71.6 % inhibition) and antiviral (98 % inhibition of ZikV) activities were observed for the extract of Fusarium verticillioides, suggesting that papain can be an effective model for discovering compounds with therapeutic potential in protease-mediated diseases. Antifungal activity against Candida spp. was also observed in extracts cultivated on corn, specifically those from Fusarium petroliphilum and F. sacchari. Overall, these results highlight the biotechnological and pharmacological potential of fruit-associated fungi, opening new perspectives for the discovery of novel bioactive natural compounds.

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探索果实相关真菌的生物活性潜力：天然提取物的抗真菌、抗寄生虫和抗病毒活性。

本研究调查了与富含蛋白酶的水果（如木瓜和菠萝）相关的真菌作为生物活性提取物来源的潜力。真菌菌株在不同培养基（水稻、玉米、小麦、燕麦和甘蔗渣）中培养。所得提取物通过1H NMR进行化学分析，可以观察到根索拉尼醇、血红素、beauvericin、环孢素和镰刀二酸等化合物。在水稻上培养的提取物表现出较高的木瓜蛋白酶抑制活性，其中尤以Neofusicoccum ribis（在50 μg/mL下抑制99.4% %）和sacchari镰刀菌（在50 μg/mL下抑制89.6% %）为显著。此外，黄萎病镰刀菌提取物具有良好的抗锥虫活性（抑制71.6 %）和抗病毒活性（抑制98 %），表明木瓜蛋白酶可以作为发现蛋白酶介导疾病中具有治疗潜力的化合物的有效模型。在玉米上培养的镰刀菌提取物对念珠菌也有一定的抑菌活性，其中石油镰刀菌提取物和糖化镰刀菌提取物的抑菌活性最强。总之，这些结果突出了果实相关真菌的生物技术和药理潜力，为发现新的生物活性天然化合物开辟了新的视角。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.