Combined chronic oral methylphenidate and fluoxetine treatment increases CB1 receptor density in the somatosensory forelimb region

IF 2.2 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-12-10 Epub Date: 2025-10-02 DOI:10.1016/j.neulet.2025.138407

Abigail M. Lantry , Huy Lu , Matt Marion , John Hamilton , Brittany Richardson , Teresa Quattrin , Lucy D. Mastrandrea , Michael Hadjiargyrou , David Komatsu , Panayotis K. Thanos

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引用次数: 0

Abstract

Methylphenidate (MP) is a psychostimulant commonly prescribed for attention-deficit/hyperactivity disorder (ADHD), and Fluoxetine (FLX) is a selective serotonin reuptake inhibitor (SSRI) commonly prescribed to treat depression and anxiety disorders. Both are shown to impact neurochemistry and behavior, but little is known about their individual and combined impacts on the endocannabinoid system (ECS). We examined the effects of MP and FLX, both as separate treatments and in combination, on cannabinoid receptor type 1 (CB1) binding in several key brain regions of interest. Male rats were treated with either water, MP, FLX, or MP + FLX via a previously established drinking paradigm for three months. Brains were harvested, and [³H] SR141716A in vitro autoradiography was performed to quantify CB1 binding. The combined treatment of MP + FLX showed significantly higher [³H] SR141716A binding compared to the water, MP, and FLX groups in the somatosensory forelimb (S(FL)) region. This indicates an ability of the common co-usage of MP and FLX to increase CB1 levels in the somatosensory cortex: a region of the brain required for the processing of sensory information.

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慢性口服哌甲酯和氟西汀联合治疗可增加躯体感觉前肢区域CB1受体密度。

哌醋甲酯（MP）是一种精神兴奋剂，通常用于治疗注意力缺陷多动障碍（ADHD），氟西汀（FLX）是一种血清素选择性再摄取抑制剂（SSRI），通常用于治疗抑郁症和焦虑症。两者都被证明会影响神经化学和行为，但对它们对内源性大麻素系统（ECS）的单独和联合影响知之甚少。我们研究了MP和FLX的作用，无论是单独治疗还是联合治疗，对大麻素受体1型（CB1）在几个关键脑区结合的影响。雄性大鼠分别用水、MP、FLX或MP + FLX通过先前建立的饮用模式治疗三个月。采集脑，对[3H] SR141716A进行体外放射自显影以定量CB1结合。与水、MP和FLX组相比，MP + FLX联合处理在体感觉前肢S（FL）区显示出更高的[3H] SR141716A结合。这表明MP和FLX的共同使用能够增加体感觉皮层（大脑中处理感觉信息所需的区域）中CB1的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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