Mandibular Advancement Device Improves the Function of Hypoglossal Nerve in OSA Rabbits.

IF 4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of oral rehabilitation Pub Date : 2025-10-03 DOI:10.1111/joor.70060

Xing Qiao, Dechao Zhu, Xiaoyi Wang, Chunyan Liu, Zuo Zhang, Peipei Zhang, Haiyan Lu

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引用次数: 0

Abstract

Background: This study investigates the interaction between the motor endplate of the genioglossus and the hypoglossal nerve in an obstructive sleep apnea (OSA) model and explores the mechanism of mandibular advancement devices (MAD) in treating OSA.

Methods: 18 male New Zealand white rabbits were randomly divided into three groups-control, OSA and MAD (n = 6 each). OSA was induced in the OSA and MAD groups by injecting gel into the soft palate. The MAD group received MAD treatment. Cone-beam computed tomography (CBCT) and polysomnography (PSG) confirmed MAD efficacy. Rabbits underwent 2 h of sleep induction daily for 8 weeks. Electrophysiological signals of the genioglossus and hypoglossal nerve were recorded. Morphological assessments of nerve fibres and motor endplates used gold chloride, acetylcholine and haematoxylin-Eosin (HE) staining. Immunofluorescence and acetylcholinesterase (AChE) staining evaluated nerve conduction.

Results: OSA symptoms were successfully induced and reversed by MAD. Structural and functional impairments of the hypoglossal nerve and motor endplates in the OSA group were alleviated by MAD, improving nerve impulse conduction and muscle function.

Conclusions: MAD treatment mitigates OSA-induced damage to the hypoglossal nerve and genioglossus motor endplates, enhancing structure and function.

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下颌推进装置对OSA兔舌下神经功能的改善。

背景：本研究探讨阻塞性睡眠呼吸暂停（OSA）模型中颏舌肌运动终板与舌下神经的相互作用，并探讨下颌推进装置（MAD）治疗OSA的机制。方法：雄性新西兰大白兔18只，随机分为对照组、OSA组和MAD组，每组6只。通过软腭注射凝胶诱导OSA组和MAD组的OSA。MAD组接受MAD治疗。锥束计算机断层扫描（CBCT）和多导睡眠图（PSG）证实了MAD的疗效。连续8周，每天诱导睡眠2小时。记录颏舌肌和舌下神经的电生理信号。神经纤维和运动终板的形态学评估采用氯化金、乙酰胆碱和苏木精-伊红（HE）染色。免疫荧光和乙酰胆碱酯酶（AChE）染色评估神经传导。结果：MAD成功诱导并逆转了OSA症状。OSA组舌下神经和运动终板的结构和功能损伤均得到缓解，神经冲动传导和肌肉功能得到改善。结论：MAD治疗可减轻osa引起的舌下神经和颏舌肌运动终板损伤，增强结构和功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral rehabilitation 医学-牙科与口腔外科

CiteScore

5.60

自引率

10.30%

发文量

116

审稿时长

4-8 weeks

期刊介绍： Journal of Oral Rehabilitation aims to be the most prestigious journal of dental research within all aspects of oral rehabilitation and applied oral physiology. It covers all diagnostic and clinical management aspects necessary to re-establish a subjective and objective harmonious oral function. Oral rehabilitation may become necessary as a result of developmental or acquired disturbances in the orofacial region, orofacial traumas, or a variety of dental and oral diseases (primarily dental caries and periodontal diseases) and orofacial pain conditions. As such, oral rehabilitation in the twenty-first century is a matter of skilful diagnosis and minimal, appropriate intervention, the nature of which is intimately linked to a profound knowledge of oral physiology, oral biology, and dental and oral pathology. The scientific content of the journal therefore strives to reflect the best of evidence-based clinical dentistry. Modern clinical management should be based on solid scientific evidence gathered about diagnostic procedures and the properties and efficacy of the chosen intervention (e.g. material science, biological, toxicological, pharmacological or psychological aspects). The content of the journal also reflects documentation of the possible side-effects of rehabilitation, and includes prognostic perspectives of the treatment modalities chosen.