Enhancement of Corchorus olitorius L. on osteogenic differentiation of MC3T3-E1 pre-osteoblast cells by increasing alkaline phosphatase and hydroxyproline.

IF 1.6 3区 化学 Q3 CHEMISTRY, APPLIED
Pinar Ertugruloglu, Elif Baris, Gaye Sumer Okkali, Nazli Boke Sarikahya
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引用次数: 0

Abstract

Corchorus olitorius L. (jute mallow or molehiya) belongs to the Malvaceae family valued for its nutritional and medicinal properties. In this study, the potential to enhance osteogenesis in MC3T3-E1(Murine Calvaria-derived 3T3 Subclone E1) pre-osteoblastic cells was investigated to support bone formation and mineralisation. Leaf ethanolic extract was prepared and applied to MC3T3-E1 cells. Osteogenic effects were evaluated through three methods: MTT assays for cell viability, Alizarin Red S staining for calcium deposition, enzymatic analyses for alkaline phosphatase (ALP) and hydroxyproline (HYP). A non-cytotoxic concentration of C. olitorius extract (0.5 mg/mL) significantly increased ALP and HYP levels, promoting osteogenic differentiation in both undifferentiated and differentiated cells. HYP levels were notably elevated in differentiated cells. The findings suggested that C. olitorius extract may be a promising natural agent for enhancing bone health, warranting further in vivo and clinical studies to confirm its therapeutic potential.

通过增加碱性磷酸酶和羟脯氨酸对MC3T3-E1前成骨细胞成骨分化的促进作用。
黄麻锦葵(Corchorus olitorius L.)属于锦葵科,因其营养和药用价值而受到重视。在这项研究中,研究人员研究了MC3T3-E1(小鼠calvaria衍生的3T3亚克隆E1)成骨前细胞促进骨形成和矿化的潜力。制备叶片乙醇提取物,并应用于MC3T3-E1细胞。通过MTT法测定细胞活力,茜素红S染色测定钙沉积,酶促法测定碱性磷酸酶(ALP)和羟脯氨酸(HYP)来评价成骨作用。无细胞毒性浓度的石竹提取物(0.5 mg/mL)可显著提高ALP和HYP水平,促进未分化和分化细胞的成骨分化。分化细胞中HYP水平明显升高。本研究结果提示,山茱萸提取物可能是一种很有前景的天然增强骨健康剂,需要进一步的体内和临床研究来证实其治疗潜力。
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来源期刊
Natural Product Research
Natural Product Research 化学-医药化学
CiteScore
5.10
自引率
9.10%
发文量
605
审稿时长
2.1 months
期刊介绍: The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
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