PLK1 inhibition enhances gemcitabine-induced apoptosis through PLK1-dependent ERK1/2-Bim and AKT1/Noxa signals in pancreatic cancer cells.

Abstract

Polo-like kinase 1 (PLK1) is a critical regulator of many cell cycle events, which has been found to be associated with resistance to cytotoxic drugs. In the present study, we investigates how PLK1 regulates the sensitivity of pancreatic cancer cells to Gemcitabine (GEM) and its mechanism. We detected the expression of PLK1 in pancreatic cancer tissues and cell lines and study the effects of PLK1 and Gemcitabine on cell viability and apoptosis of GEM-resistant pancreatic cancer PANC-1 cells and Gemcitabine sensitive BxPC-3 cells; Using inhibitors or siRNA, we further investigate the effects of PLK1 on ERK1/2, AKT1, and pro-apoptotic genes PUMA, Bim, and Noxa; We finally investigated the effect of the combined onvansertib and Gemcitabine on the growth of PANC-1 subcutaneous transplant tumors in nude mice and explored its possible mechanism of action. PLK1 is overexpressed in PDAC tissues and cell lines. Targeting PLK1 inhibits cell viability and induces cell apoptosis in PANC-1 cells in vitro by ERK1/2-dependent Bim and AKT1-dependent Noxa pathway. Gemcitabine activates ERK1/2 and AKT1, leading to acquired Gemcitabine resistance. Targeting PLK1 enhances Gemcitabine sensitivity in PANC-1 cells by upregulating ERK1/2-dependent Bim and AKT1-dependent Noxa expression in vitro. PLK1 re-expression by PLK1 transfection in PLK1 shRNA transfected PANC-1 cells recures Gemcitabine sensitivity by inhibiting ERK1/2-dependent Bim and AKT1-dependent Noxa expression in vitro. Enhanced PLK1 reduces Gemcitabinecitabine sensitivity in BxPC-3 cells by inhibiting Gemcitabine-induced Bim and Noxa expression. BI2536 (a PLK1 kinase inhibitor) treatment recures the Gemcitabine sensitivity in the PLK -transfected BxPC-3 cells by upregulation of Bim and Noxa expression in vitro. The combination of onvansertib and Gemcitabine showed significant anti-tumor effect in vivo. Mechanistically, combined treatment inhibits ERK1/2 and AKT1, and increases Bim and Noxa expression. Targeting PLK1 sensitizes PDAC cells to gemcitabine in vitro and in vivo. This indicates that combination therapy with PLK1 inhibitor may overcome gemcitabine resistance, offering a promising new therapeutic option for the treatment of gemcitabine-resistant human pancreatic cancer.