The GARP-ARR-B family transcription factor SeAPRR2 positively regulates drought tolerance in chayote (Sechium edule)

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-26 DOI:10.1016/j.plaphy.2025.110561

Shaobo Cheng, Zhili Chen, Wei Yan, Lihong Su, Xiaoting Zhou, Zhongqun He

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引用次数: 0

Abstract

Chayote, an economically vital cucurbit crop, faces severe production and quality constraints due to increasing drought stress. Despite this threat, systematic identification and analysis of the GARP-ARR-B transcription factor family in chayote remain unreported. This study identified 11 ARR-B transcription factors in chayote, with focused investigation on the drought-responsive gene SeAPRR2. SeAPRR2 exhibited rapid induction under PEG-simulated drought, and its heterologous expression significantly enhanced osmotic stress tolerance in yeast. Furthermore, SeAPRR2 overexpression in tomato substantially improved drought resistance, manifested through reduced wilting, decreased electrolyte leakage, lower malondialdehyde content, elevated proline accumulation, attenuated reactive oxygen species (ROS), and accelerated stomatal closure. The interaction between SeAPRR2 and the metallochaperone SeHIPP3 was confirmed by Y2H, LCI, BIFC and molecular docking. Additionally, DAP-seq, Y1H, and LUC assays demonstrated SeAPRR2's direct binding and transcriptional activation of SeP5CS1 and SeNCED5. Collectively, SeAPRR2 synchronously enhances drought tolerance through proline-mediated osmotic adjustment and ABA-triggered stomatal closure, suggesting its potential as a candidate gene for enhancing drought tolerance in chayote germplasm.

查看原文本刊更多论文

GARP-ARR-B家族转录因子SeAPRR2正调控佛手瓜（Sechium edule）的耐旱性。

佛手瓜是一种经济上至关重要的瓜类作物，由于日益严重的干旱胁迫，佛手瓜面临严重的产量和质量限制。尽管存在这种威胁，但佛手瓜中GARP-ARR-B转录因子家族的系统鉴定和分析仍未见报道。本研究鉴定了佛手瓜中11个ARR-B转录因子，重点研究了干旱响应基因SeAPRR2。SeAPRR2在peg模拟干旱条件下被快速诱导，其异源表达显著提高了酵母的渗透胁迫耐受性。此外，SeAPRR2在番茄中的过表达显著提高了番茄的抗旱性，表现为减少萎蔫、减少电解质泄漏、降低丙二醛含量、增加脯氨酸积累、减弱活性氧（ROS）和加速气孔关闭。通过Y2H、LCI、BIFC和分子对接证实了SeAPRR2与金属伴侣蛋白SeHIPP3的相互作用。此外，DAP-seq、Y1H和LUC检测显示SeAPRR2直接结合并转录激活SeP5CS1和seenced5。综上所述，SeAPRR2通过脯氨酸介导的渗透调节和aba触发的气孔关闭同步增强了佛手瓜种质的耐旱性，表明其可能是增强佛手瓜种质抗旱性的候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.