Electrophoresis-based electrochemical sensor for rapid detection of sialic acid in human serum

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-10-04 DOI:10.1007/s00604-025-07551-w

Xiaoqing Ming, Zhanghong Wu, Shuluo Yin, Xiangyu Zuo, Qing Lu, Xiaoting Wu, Xinhang Li, Shengnan Tang, Fangxin Liu, Yuanfang Hou, Jianjun Hu, Guangchao Zang

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Abstract

A novel electrochemical sensor is introduced that significantly accelerates sialic acid (SA) detection by utilizing an electrophoresis device, which reduces the binding time from 20 to 5 min. The results indicate that the current signal change (ΔI) of the sensor is more than twofold higher than that of conventional sensors after 5 min. Furthermore, to enhance the intensity of the sensor’s signal and simplify the operational procedure, we employed a method that electrochemically reduces graphene oxide/3-aminophenylboronic acid (GO/APBA) to reduced graphene oxide/3-aminophenylboronic acid (rGO/APBA) in a single step. This reduction process effectively removes some oxygen groups from GO, further enhancing the current signal. The linear detection range for SA was between 0.1 mM and 3 mM, with a detection limit of 0.084 mM (S/N = 3). Notably, the electrophoresis-enhanced sensor can successfully detect SA in actual human serum samples. Overall, this sensor represents a significant advancement in the electrochemical sensing of SA, providing a rapid, selective, and cost-effective approach for developing electric field-enhanced detection technologies.

Graphical Abstract

查看原文本刊更多论文

快速检测人血清唾液酸的电泳电化学传感器。

介绍了一种新型的电化学传感器，该传感器利用电泳装置显著加速了唾液酸（SA）的检测，将结合时间从20分钟缩短到5分钟。结果表明，在5min后，传感器的电流信号变化（ΔI）比传统传感器高两倍以上。此外，为了增强传感器的信号强度并简化操作程序，我们采用了电化学一步还原氧化石墨烯/3-氨基苯基硼酸（GO/APBA）为还原氧化石墨烯/3-氨基苯基硼酸（rGO/APBA）的方法。这种还原过程有效地去除氧化石墨烯中的一些氧基，进一步增强电流信号。SA的线性检测范围为0.1 mM ~ 3 mM，检出限为0.084 mM （S/N = 3）。值得注意的是，电泳增强传感器可以成功检测实际人血清样品中的SA。总的来说，该传感器代表了SA电化学传感的重大进步，为开发电场增强检测技术提供了快速，选择性和成本效益的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.