LC–MS/MS–Based Serum Lipidomic Profile to Reveal Cancer-Specific Biomarkers for Renal Cell Carcinoma

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-10-04 DOI:10.1002/bmc.70230

Lin Lin, Jiaxin Zheng, Sixia Hu

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Abstract

Renal cell carcinoma (RCC) is one of the most lethal malignant tumors in the urinary system, and its incidence rate is increasing in the last decade. Discovering sensitive and specific biomarkers for RCC and exploring its pathogenesis are of great clinical significance. Here, a liquid chromatography–tandem mass spectrometry (LC–MS/MS) based serum lipidomic profile was carried out to reveal the lipid expression differences in RCC patients and healthy controls. A total of 112 participants were enrolled in the training set, including nonmalignant disease subjects for specific screening. As a result, 66 RCC-specific lipid variables were screened and further validated with an independent test set. Receiver operating characteristic (ROC) analysis was used to evaluate the clinical diagnostic potential of the biomarkers. Twenty-four of them were found to have AUC (area under the curve) values larger than 0.85, and 13 of them were clearly identified. The study indicated reduced lysophosphatidylcholine (LPC) serum levels and increased triglyceride (TG) levels in RCC patients, which might be developed as a potential therapeutic target for cancer treatment. A biomarker pattern composed of TG (19:1/18:2/18:2) and TG (18:0/16:0/16:0) was discovered to have the highest AUC value of 0.995, with a sensitivity and specificity up to 97.6% and 95.0%, respectively.

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LC-MS / MS-Based血清脂质组学分析揭示肾细胞癌的癌症特异性生物标志物

肾细胞癌（RCC）是泌尿系统最致命的恶性肿瘤之一，近十年来其发病率呈上升趋势。发现敏感特异的RCC生物标志物，探索其发病机制具有重要的临床意义。本研究采用液相色谱-串联质谱法（LC-MS /MS）进行血清脂质组学分析，以揭示RCC患者和健康对照组的脂质表达差异。共有112名参与者被纳入训练集，包括非恶性疾病受试者进行特定筛查。结果，筛选了66个rcc特异性脂质变量，并通过独立测试集进一步验证。使用受试者工作特征（ROC）分析来评估生物标志物的临床诊断潜力。其中24个曲线下面积（AUC）大于0.85，其中13个得到了明确的鉴定。该研究提示RCC患者血清溶血磷脂酰胆碱（LPC）水平降低，甘油三酯（TG）水平升高，可能成为癌症治疗的潜在靶点。发现由TG（19:1/18:2/18:2）和TG（18:0/16:0/16:0）组成的生物标志物模式AUC值最高，为0.995，敏感性和特异性分别达到97.6%和95.0%。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.