Excitable temperament impairs oocyte quality and in vitro embryo production efficiency in Nellore donors

IF 1.9 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Livestock Science Pub Date : 2025-10-02 DOI:10.1016/j.livsci.2025.105827

Samuel Rodrigues Bonamichi do Couto , Nicolas Moreira Piedras Monnerat Caparelli , Thiago Souza Vieira , Daniela Cristina Rocha de Freitas , Mateus Gonçalves Costa , Mellyssa Sad Rodrigues Gomes , João Paulo Nascimento Andrade , Rondineli Pavezzi Barbero , Felipe Zandonadi Brandão , Marco Roberto Bourg de Mello

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Abstract

This study aimed to evaluate the effects of temperament and reactivity on oocyte recovery and in vitro embryo production (IVEP) efficiency in Nellore females. We hypothesized that stress from an excitable temperament would impair IVEP outcomes. This study was conducted on a commercial farm in Paraná, Brazil, involving 133 Nellore females, yielding 3,820 oocytes. Temperament was scored on a scale of 1–4 based on behavior during handling, categorizing animals into calm (ADQ; score ≤ 2) or excitable (EXC; score > 2) groups. Cortisol concentrations were measured to validate the temperament assessments. Oocytes were morphologically evaluated and subjected to a commercial IVEP protocol. Statistical analyses were performed using the GLIMMIX procedure in SAS (P ≤ 0.05). Excitable females (n = 32) had higher cortisol levels than calm ones (n = 101) (62.5 ± 7.2 vs. 42.2 ± 3.8 ng/mL; P = 0.008). The ADQ group had a higher proportion of viable oocytes (79.3%¦vs. 75.5%; P = 0.01) and lower degeneration rates (20.6%¦vs. 24.4%; P = 0.01). The cleavage (74.9%¦vs. 64.7%; P < 0.001) and blastocyst (25.4%¦vs. 19.2%; P = 0.02) rates were also higher in calm females, with greater blastocyst production per donor (6.2 vs. 3.0 embryos; P < 0.001). In conclusion, excitable temperament negatively affects oocyte quality and IVEP efficiency, suggesting that temperament-based selection and management can enhance IVEP success in Nellore cattle.

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易激动的性情会损害Nellore供体的卵母细胞质量和体外胚胎产生效率

本研究旨在探讨气质和反应性对Nellore雌性卵母细胞恢复和体外胚胎产生（IVEP）效率的影响。我们假设易激动的性情所带来的压力会损害IVEP的结果。这项研究是在巴西paran的一个商业农场进行的，涉及133只Nellore雌性，产生3820个卵母细胞。根据处理过程中的行为，将动物分为冷静组（ADQ；得分≤2）和兴奋组（EXC；得分>； 2），性情评分为1-4分。测量皮质醇浓度以验证气质评估。卵母细胞进行形态学评估，并进行商业IVEP方案。SAS采用GLIMMIX程序进行统计学分析（P≤0.05）。兴奋女性（n = 32）的皮质醇水平高于平静女性（n = 101）（62.5±7.2∶42.2±3.8 ng/mL; P = 0.008）。ADQ组存活卵母细胞比例较高（79.3%）。75.5%;P = 0.01)和较低的退变率（20.6%）。24.4%;P = 0.01)。卵裂度（74.9%）。64.7%;P < 0.001)和囊胚（25.4%）。19.2%;P = 0.02)，平静雌性的比例也更高，每个供体的囊胚产量更高（6.2比3.0个胚胎；P < 0.001）。综上所述，易兴奋性气质对卵母细胞质量和IVEP效率有负向影响，表明基于气质的选择和管理可以提高内洛尔牛IVEP的成功率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Livestock Science 农林科学-奶制品与动物科学

CiteScore

4.30

自引率

5.60%

发文量

237

审稿时长

3 months

期刊介绍： Livestock Science promotes the sound development of the livestock sector by publishing original, peer-reviewed research and review articles covering all aspects of this broad field. The journal welcomes submissions on the avant-garde areas of animal genetics, breeding, growth, reproduction, nutrition, physiology, and behaviour in addition to genetic resources, welfare, ethics, health, management and production systems. The high-quality content of this journal reflects the truly international nature of this broad area of research.