Exploring the medicinal potential of in vitro cultures for enhanced production of metabolite empowered by green silver nanoparticles in Alhagi maurorum medik

Abstract

Alhagi maurorum, a valuable medicinal plant, presents an opportunity for sustainable biomass production and amplification of therapeutic compounds. This study explores the use of biologically synthesized silver nanoparticles (AgNPs) in plant tissue culture to enhance secondary metabolite production. In vitro cultures were established using modified Murashige and Skoog medium supplemented with growth regulators and adjuvants. AgNPs significantly promoted callus formation and differentiation. Notably, 8.0 mg/L AgNPs enhanced protein, phenolic, and flavonoid contents and antioxidant enzyme activities. HPLC profiling confirmed the presence of lupeol in AgNP-treated cultures. These findings indicate the potential of nanobiotechnology in boosting the medicinal value of in vitro plant cultures.