Polarization resolved deep ultraviolet microscopy for label free imaging with enhanced nuclei and fiber contrast

IF 3.7 2区工程技术 Q2 OPTICS

Optics and Lasers in Engineering Pub Date : 2025-10-01 DOI:10.1016/j.optlaseng.2025.109375

Jiabin Chen , Ruilin You , Marco Contreras , Haijiang Cai , Yuanyuan Sun , Yihan Wang , Bofan Song , Stanley Pau , Zhihan Hong , Rongguang Liang

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引用次数: 0

Abstract

Deep ultraviolet (DUV) microscopy is a rapid, label-free imaging technique widely used in biological applications. However, fibrillar structures, which provide crucial insights into tissue organization, are often overlooked. In this work, we introduce a polarization-resolved DUV microscope capable of extracting both nuclear and fiber features. Illumination at 265 nm enhances nuclear contrast, while polarization imaging reveals fiber orientation. Four images are captured sequentially to calculate the linear polarization properties of the tissue medium. To address pixel misalignment between multiple images, we apply adaptive local thresholds to extract valid features for precise registration. The degree of linear polarization and angle of polarization exhibit significant changes as light passes through tissue samples, revealing variations in polarization states. This additional polarization contrast offers a new dimension of analysis, potentially enhancing the characterization of biological tissues.

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偏振分辨深紫外显微镜用于无标签成像与增强核和纤维对比

深紫外显微技术是一种快速、无标记的成像技术，广泛应用于生物领域。然而，为组织结构提供重要见解的纤维结构经常被忽视。在这项工作中，我们介绍了一个偏振分辨DUV显微镜能够提取核和纤维的特征。265 nm的光照增强了核的对比度，而偏振成像显示了纤维的取向。连续捕获四幅图像以计算组织介质的线偏振特性。为了解决多幅图像之间的像素不对齐问题，我们应用自适应局部阈值提取有效特征进行精确配准。当光通过组织样品时，线偏振度和偏振角发生显著变化，揭示了偏振态的变化。这种额外的极化对比提供了一个新的分析维度，潜在地增强了生物组织的表征。

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来源期刊

Optics and Lasers in Engineering 工程技术-光学

CiteScore

8.90

自引率

8.70%

发文量

384

审稿时长

42 days

期刊介绍： Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods. Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following: -Optical Metrology- Optical Methods for 3D visualization and virtual engineering- Optical Techniques for Microsystems- Imaging, Microscopy and Adaptive Optics- Computational Imaging- Laser methods in manufacturing- Integrated optical and photonic sensors- Optics and Photonics in Life Science- Hyperspectral and spectroscopic methods- Infrared and Terahertz techniques