Unravelling the microbial ecosystem based on core environmental factors and bacteria dynamics during cyanobacteria powder amended pig manure co-composting

Abstract

This study reveals the effect of cyanobacteria dehydrated powder (CDP) amendment on core environmental factors and bacteria dynamics during the pig manure (PM) composting. The composting was carried out with the participation of six different concentrations of CDP mixed with PM+ wheat straw (0 %, 2.5 %, 5 %, 7.5 %, 10 %, 20 %, and marked T1 to T6, respectively). Results indicated that the succession of bacterial abundance was first slightly decline and then steady, where the three dominant phyla, Firmicutes (30.54–74.75 %), Bacteroidota (3.8–33.84 %) and Proteobacteria (8.14–19.75 %) were noticed. CDP amendment was increased the abundance of Sinibacillus (Max. 5.91 times), and important role play in the humification process. Organic matter-degrading bacterial genus Bacillus (1.33–2.9 times) and Fermentimonas (2.07–6.02 times) were further activated on the basis of indigenous microbial succession. The increase of β-diversity was showed that CDP as a sort of biomass mainly working after mesophilic phase. The highest P/N value of 2.78 and the largest number of nodes and edges (243 nodes/ 2860 edges) of T4 have been recorded by co-occurrence network. Mantel test show that the three dominant phyla have complex correlation with environmental factors on biochar applied treatments. CDP amended PM co-composting promotes the abundance of mature bacterial genera Fermentimonas, and reflects its promising role in mature stage.