Sustainable biodiesel production from slaughter byproduct fat using zirconia–lipase cluster

IF 7.8 2区环境科学与生态学 Q1 ENGINEERING, CHEMICAL

Process Safety and Environmental Protection Pub Date : 2025-09-30 DOI:10.1016/j.psep.2025.107942

Elizabeth Olufunmilayo Oladepo , Gwanyeong Ko , Jun Seop Lee , Hye Sun Lee , Ee Taek Hwang

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引用次数: 0

Abstract

Refined slaughter byproduct fat is obtained through the thermal and mechanical processing of animal waste generated during slaughtering and meat processing. The utilization of these non-edible fats and oils as feedstocks for biodiesel production is highly beneficial since it does not compete with the food industry and contributes to global waste management by reducing waste. This study aimed to stabilize lipase from Pseudomonas fluorescens by immobilizing it with zirconia to form a zirconia-lipase cluster and applying it in the transesterification of waste fat from refined slaughter byproduct to produce biodiesel in a solvent-free medium. The immobilized lipase demonstrated hyperactivated hydrolysis activity, with 125 % higher specific hydrolysis activity than free lipase did, and 54 % of the initial activity of the immobilized lipase was retained after 100 days of storage at room temperature. The kinetic parameters from the kinetic study indicated that compared with free lipase, zirconialipase clusters significantly improved substrate affinity and catalytic efficiency on the basis of its lower k_m and higher V_max·K_m⁻¹. Additionally, in a solvent-free transesterification system, the zirconialipase clusters achieved a biodiesel yield that was 4.16 times greater than that of the free lipase. To the best of our knowledge, this is the first report on the conversion of refined slaughter byproduct fat into biodiesel via a solvent-free reaction catalyzed by hyperactive zirconia–lipase clusters.

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利用氧化锆-脂肪酶簇从屠宰副产品脂肪中可持续生产生物柴油

精制屠宰副产品脂肪是通过对屠宰和肉类加工过程中产生的动物粪便进行热加工和机械加工而获得的。利用这些非食用脂肪和油作为生物柴油生产的原料是非常有益的，因为它不与食品工业竞争，并通过减少浪费为全球废物管理做出贡献。本研究旨在通过氧化锆固定荧光假单胞菌脂肪酶，形成氧化锆-脂肪酶簇，并将其应用于精制屠宰副产物的废脂肪在无溶剂培养基中酯交换生产生物柴油。固定化脂肪酶表现出超激活的水解活性，比游离脂肪酶的特异性水解活性高125 %，在室温下保存100天后，固定化脂肪酶的初始活性保持在54 %。动力学参数表明，与游离脂肪酶相比，锆脂酶簇具有较低的km和较高的Vmax·km−1，显著提高了底物亲和力和催化效率。此外，在无溶剂酯交换系统中，锆脂酶簇获得的生物柴油产量是游离脂肪酶的4.16倍。据我们所知，这是第一篇关于通过超活性氧化锆-脂肪酶簇催化的无溶剂反应将精制屠宰副产物脂肪转化为生物柴油的报道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Process Safety and Environmental Protection 环境科学-工程：化工

CiteScore

11.40

自引率

15.40%

发文量

929

审稿时长

8.0 months

期刊介绍： The Process Safety and Environmental Protection (PSEP) journal is a leading international publication that focuses on the publication of high-quality, original research papers in the field of engineering, specifically those related to the safety of industrial processes and environmental protection. The journal encourages submissions that present new developments in safety and environmental aspects, particularly those that show how research findings can be applied in process engineering design and practice. PSEP is particularly interested in research that brings fresh perspectives to established engineering principles, identifies unsolved problems, or suggests directions for future research. The journal also values contributions that push the boundaries of traditional engineering and welcomes multidisciplinary papers. PSEP's articles are abstracted and indexed by a range of databases and services, which helps to ensure that the journal's research is accessible and recognized in the academic and professional communities. These databases include ANTE, Chemical Abstracts, Chemical Hazards in Industry, Current Contents, Elsevier Engineering Information database, Pascal Francis, Web of Science, Scopus, Engineering Information Database EnCompass LIT (Elsevier), and INSPEC. This wide coverage facilitates the dissemination of the journal's content to a global audience interested in process safety and environmental engineering.