Electrospun and freeze-dried PCL/chitosan-gelatin bilayer dressing for enhanced wound healing

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-09-26 DOI:10.1016/j.carpta.2025.101016

Mehdi Jalili Ahmadabad , Mahsa Janmohammadi , Zahra Nazemi , Marjan Bahraminasab , Samaneh Arab , Mohammad Sadegh Nourbakhsh

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Abstract

A double-layer (DL) wound dressing was developed by integrating electrospinning and freeze-drying techniques. The electrospun top layer (PCL/Cs) provided hydrophobicity and resistance to bacterial penetration. The incorporation of 5 % Cs increased the tensile strength of nanofibers from 5.4 ± 2.26 MPa to 12.9 ± 2.23 MPa. The freeze-dried bottom layer (Cs/Gel) exhibited a high swelling ratio exceeding 500 %, and compressive properties ranging from 0.35 to 1.36 MPa. The water vapor transmission rate (WVTR) for Cs-Gel single layers was within the range of 2000–2500 g/m²/day, while DL-50Cs and DL-25Cs exhibited WVTRs of 3300 ± 110 and 1993 ± 136 g/m²/day, respectively. The samples showed antibacterial activity against Staphylococcus aureus (S. aureus (Cs25-Gel75 (82 %), Cs50-Gel50 (72 %), DL-50Cs (78 %), DL-25Cs (79 %), PCL-Cs5 % (41 %)). The MTT revealed high cell viability for all samples, with DL-25Cs showing 453 ± 111 % viability after 3 days and 201 ± 12 % viability after 7 days. The scratch assay on L929 cells showed enhanced wound closure for all samples compared with the control (16 ± 11 %), Cs25-Gel75 (18 ± 6 %), Cs50-Gel50 (19 ± 10 %), DL-50Cs (21 ± 9 %), and DL-25Cs (23 ± 5 %). DL-25Cs exhibited the highest closure, indicating improved cell migration potential due to the double-layer structure.

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电纺丝和冻干PCL/壳聚糖-明胶双层敷料促进伤口愈合

将静电纺丝和冷冻干燥技术相结合，研制了一种双层创面敷料。静电纺顶层（PCL/Cs）具有疏水性和抗细菌渗透能力。5% Cs的掺入使纳米纤维的抗拉强度由5.4±2.26 MPa提高到12.9±2.23 MPa。冻干后的底层（Cs/Gel）溶胀率超过500%，压缩性能在0.35 ~ 1.36 MPa之间。Cs-Gel单层的水蒸气透过率（WVTR）在2000 ~ 2500 g/m²/day之间，DL-50Cs和DL-25Cs的水蒸气透过率分别为3300±110和1993±136 g/m²/day。样品对金黄色葡萄球菌（S. aureus, Cs25-Gel75（82%）、Cs50-Gel50（72%）、DL-50Cs（78%）、DL-25Cs（79%）、PCL-Cs5(41%)）均有抑菌活性。MTT显示所有样品的细胞活力都很高，DL-25Cs在3天后的活力为453±111%，在7天后的活力为201±12%。L929细胞划痕实验显示，与对照组（16±11%）、Cs25-Gel75（18±6%）、Cs50-Gel50（19±10%）、DL-50Cs（21±9%）和DL-25Cs（23±5%）相比，所有样本的伤口愈合程度都有所提高。DL-25Cs表现出最高的闭合性，表明双层结构提高了细胞迁移潜力。

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来源期刊

Carbohydrate Polymer Technologies and Applications

CiteScore

8.70

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0.00%

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