Comparative evaluation of the efficiency of different decontamination protocols for reusing healing abutments: An in vitro study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-10-01 DOI:10.1016/j.jobcr.2025.09.024

Dhruv Patel, Nayana Shriram Anasane, V.N.V. Madhav

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引用次数: 0

Abstract

Purpose

Healing abutments are subjected to multiple cycles of reuse owing to the limited wear and tear experienced in function. The study undertaken focuses on drawing a comparative evaluation amongst two decontamination protocols for reusing healing abutments.

Materials and method

A total of 85 healing abutments were divided into 3 groups. Group 1 contained 5 unused healing abutments. Group 2 contained 40 used healing abutments which were subjected to ultrasonic cleaning followed by autoclaving. Group 3 also contained 40 used healing abutments which were subjected to decontamination using NaOCl followed by ultrasonic cleaning followed by autoclaving. Healing abutments from all 3 groups were then individually stained with Phloxine B stain for a duration of 1 min and were individually observed under 10X Stereomicroscope for traces of staining which indicated residual contamination.

Statistical analysis

ANOVA test and chi-square test were employed to statistically analyze the 3 groups using SPSS software.

Result

The difference between the 3 groups was statistically significant (p < 0.001) with a chi-Square value of 85 %. Intergroup Comparison of mean rank showed statistically significant difference between the groups (Kruskal Wallis ANOVA, p < 0.001). Groups 1 and 3 did not display any evidence of staining with Phloxine B indicating no residual contamination, whereas all samples belonging to Group 2 displayed biological remnants as evidenced by the presence of staining.

Conclusion

Cleaning of used healing abutments using Sodium hypochlorite followed by ultrasonic cleaning and autoclaving shows greater efficiency in ensuring complete decontamination of used healing abutments as compared to ultrasonic cleaning and autoclaving.

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重复使用愈合基台的不同去污方案效率的比较评价：一项体外研究

目的修复基牙由于其功能的有限磨损，需要经历多次重复使用。所进行的研究侧重于在重复使用愈合基台的两种去污方案之间进行比较评估。材料与方法85个愈合基台分为3组。组1包含5个未使用的愈合基牙。第2组40个使用过的愈合基牙，超声清洗后进行高压灭菌。第三组40个使用过的愈合基牙，用NaOCl去污，然后超声清洗，然后高压灭菌。3组愈合基牙分别用Phloxine B染色1 min，在10倍体视显微镜下观察是否有残留污染的染色痕迹。统计学分析采用方差分析和卡方检验，采用SPSS软件对3组进行统计学分析。结果3组间差异有统计学意义（p < 0.001），卡方值为85%。组间平均秩比较显示组间差异有统计学意义（Kruskal Wallis ANOVA, p < 0.001）。第1组和第3组没有显示出任何污染残留的证据，而第2组的所有样品都显示出生物残留，这表明存在染色。结论次氯酸钠清洗后超声清洗和高压灭菌比超声清洗和高压灭菌能更有效地保证愈合基牙的完全去污。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.