Modelling and optimization of natural deep eutectic solvent-assisted extraction for carotenoids targeting lutein for developing food additives from used marigold flowers

IF 6.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2025-10-01 DOI:10.1016/j.lwt.2025.118549

Atharva Joshi, Aarya Kunnure , Abhishek Aghamkar , Amisha Garg, Tejaswini Pachpor, Nithya N. Kutty

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引用次数: 0

Abstract

Natural deep eutectic solvents (NADES) have proved their potential as a green technology for extracting phytochemicals in recent times. Different NADES combinations with various molar ratios of choline chloride as a hydrogen bond acceptor (HBA) and glucose/tartaric acid as a hydrogen bond donor (HBD) were screened for better extraction efficiency of carotenoids from a rich source-dried marigold flower. The highest carotenoid content of 549.68 μg/mg dry weight (DW) was observed in choline chloride: glucose (3:1) combination. Process optimization using Taguchi's model and response surface methodology (Box-Behnken design) was further employed to identify the optimal process parameters, viz., ultrasonication time as 35 min, incubation temperature as 25 °C, and incubation time as 2 h; a significant increase in carotenoid yield of 971.31 μg/mg DW of floral biomass was observed. Stability studies showed that carotenoids were stable in the dark and at a storage temperature of −20 °C. High performance liquid chromatography analysis revealed the presence of lutein as a major compound in the selected NADES system. Lastly, food safety studies of marigold floral NADES-based extracts were investigated. Antimicrobial and antioxidant activity studies of selected extracts revealed optimal properties indicating their potential to be formulated into food colorants and nutraceuticals.

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以叶黄素为目标的天然深共熔溶剂辅助提取类胡萝卜素的建模与优化，用于开发废弃万寿菊的食品添加剂

近年来，天然深共晶溶剂（NADES）作为一种绿色提取植物化学物质的技术已被证明具有很大的潜力。以不同摩尔比的氯化胆碱为氢键受体（HBA）和葡萄糖/酒石酸为氢键供体（HBD）为NADES组合，筛选了不同NADES组合提取富含源干金盏花中类胡萝卜素的效果。以氯化胆碱与葡萄糖（3:1）组合的类胡萝卜素含量最高，为549.68 μg/mg干重（DW）。进一步采用田口模型和响应面法（Box-Behnken设计）进行工艺优化，确定最佳工艺参数：超声时间35 min，孵育温度25℃，孵育时间2 h；花生物量类胡萝卜素产量显著增加，达971.31 μg/mg DW。稳定性研究表明，类胡萝卜素在暗处和- 20°C的储存温度下是稳定的。高效液相色谱分析表明，所选NADES体系中主要化合物为叶黄素。最后，对金盏花nades提取物的食品安全性进行了研究。所选提取物的抗微生物和抗氧化活性研究揭示了其最佳特性，表明它们有可能被配制成食品着色剂和营养保健品。

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.