Effect of freezing on mechanical properties of ovine brain tissue

IF 3.5 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2025-09-23 DOI:10.1016/j.jmbbm.2025.107213

Pavithran Devananthan , Johann Zwirner , Paul D. Docherty , Benjamin Ondruschka , Natalia Kabaliuk

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Abstract

Freezing at −20 °C impacts the mechanical properties of post mortem brain tissue, which has implications for forensic science, particularly for estimating the post mortem interval (PMI). Freezing is examined as a method that may offer an extended window for forensic analysis by halting tissue degradation processes or allow tissue transfer to specialized departments.

A rheological examination of samples from various brain regions of 21 ovine brains (frontal lobe, anterior deep brain, posterior deep brain, parietal lobe, superior colliculus, medulla, pons, cerebellum) was conducted. The brain tissue was tested on the day of sacrifice, after short term freezing (<16 days) and long-term freezing (95–110 days) to track the changes in mechanical properties.

The results showed nearly complete overlap in storage, loss and complex shear moduli between samples tested on the day of sacrifice and their frozen counterparts for all locations, with no statistically significant differences (p > 0.05) between the moduli of the three groups.

Freezing had no statistically significant impact on the tissue's mechanical properties across all tested locations. The study thus provides new insights regarding the preservation of ovine brain samples for up to 110 days for forensic biomechanical analyses.

Abstract Image

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冷冻对羊脑组织力学性能的影响

在- 20°C下冷冻会影响死后脑组织的力学特性，这对法医科学，特别是对估计死后时间间隔（PMI）有影响。冷冻是一种可以通过停止组织降解过程或允许组织转移到专门部门来提供法医分析扩展窗口的方法。对21只羊的大脑（额叶、前深脑、后深脑、顶叶、上丘、髓质、脑桥、小脑）各脑区进行了流变学检查。脑组织在祭祀当天、短期冷冻（16天）和长期冷冻（95 ~ 110天）后进行检测，追踪力学性能的变化。结果显示，在所有地点，祭祀当天测试的样品与冷冻样品的储存模量、损失模量和复合剪切模量几乎完全重合，三组之间的模量无统计学差异（p > 0.05）。在所有测试位置，冷冻对组织的机械性能没有统计学上的显著影响。因此，该研究为保存长达110天的羊脑样本进行法医生物力学分析提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.