A novel fluorescent probe for in vivo H₂S monitoring in inflammatory arthritis

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-10-01 DOI:10.1016/j.saa.2025.127007

Yihan Wang, Yifanghui Gao, Jiaqi Zhou, Yuan Jiang, Yue Wang, Cheng Zhang, Qingtao Meng, Zhiqiang Zhang

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Abstract

Hydrogen sulfide (H₂S), a crucial gaseous signaling molecule, plays a pivotal regulatory role in various physiological and pathological processes. In this study, we successfully developed a novel H₂S fluorescent probe NR based on the Nile red fluorophore. The probe achieves specific recognition of H₂S through an isothiocyanate functional group, and its recognition mechanism was confirmed by high-resolution mass spectrometry. Probe NR exhibits excellent stability and high sensitivity, with a detection limit of 3.66 μM. Demonstrating low cytotoxicity and suitability for physiological pH conditions, probe NR has been successfully applied for real-time monitoring of H₂S in vivo. In an LPS-induced arthritis model in nude mice, probe NR effectively enabled dynamic monitoring of H₂S levels at inflammatory sites. The successful development of this probe provides a powerful tool for investigating the pathophysiological mechanisms of H₂S in inflammatory diseases, demonstrating promising potential for disease diagnosis and therapeutic monitoring

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一种用于炎症性关节炎体内H₂S监测的新型荧光探针

硫化氢（H₂S）是一种重要的气体信号分子，在多种生理和病理过程中起着关键的调节作用。在这项研究中，我们成功地开发了一种基于尼罗河红荧光团的新型h2s荧光探针NR。探针通过异硫氰酸酯官能团实现对H₂S的特异性识别，并通过高分辨率质谱分析证实了其识别机制。探头NR稳定性好，灵敏度高，检测限为3.66 μM。由于具有低细胞毒性和适合生理pH条件，探针NR已成功应用于体内H₂S的实时监测。在lps诱导的裸鼠关节炎模型中，探针NR有效地实现了炎症部位H₂S水平的动态监测。该探针的成功开发为研究H₂S在炎症性疾病中的病理生理机制提供了有力的工具，在疾病诊断和治疗监测方面具有广阔的潜力

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.